|
| Status |
Public on Nov 28, 2011 |
| Title |
NSC-3 |
| Sample type |
RNA |
| |
|
| Source name |
Neural stem cell (NSC) cultured under normoxia
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain
strain: C57BL/6J x CD1
cell line: neural stem cell (NSC) line C17.2
Sex: not known if originated from male or female
tag: Immortalized with c-myc, has the LacZ as a marker/tracer
age: culture by the time of RNA extraction is 3-4 days (50% confluency)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen tissues of using RNeasy kit (Qiagen, Valencia, CA) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Illumina® TotalPrep™ RNA Amplification Kit following the manufacturer's instructions. Input amount: 100ng of total RNA.
|
| |
|
| Hybridization protocol |
Hybridizations were carried out by using the Illumina gene expression system according to the manufacturer's instructions.
|
| Scan protocol |
Illumina scanner
|
| Description |
Neural stem cell (NSC) line C17.2
|
| Data processing |
Normalized average intensity of the bead type/target in the group using Cubic spline normalization with BeadStudio.
|
| |
|
| Submission date |
Sep 13, 2010 |
| Last update date |
Nov 28, 2011 |
| Contact name |
Dan Zhou |
| E-mail(s) |
d2zhou@ucsd.edu
|
| Phone |
858-822-6889
|
| Fax |
858-534-6971
|
| Organization name |
University of California, San Diego, School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Haddad Lab
|
| Street address |
CMG 103, 9500 Gilman Drive, MC0735
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0735 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE24116 |
Identification of a neuronal gene expression signature: Role of cell-cycle arrest in murine neuronal differentiation in vitro |
|
