 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Oct 03, 2023 |
| Title |
HEK293T_WT_1 |
| Sample type |
SRA |
| |
|
| Source name |
HEK293T
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HEK293T
treatment: untreated
genotype: wild type
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent.
The library was constructed using KAPA HyperPrep RNA-seq Kit (Kapa Biosystems, KK8540).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
ADAR1_rawcount.txt
|
| Data processing |
Basecalls performed using illumina RTA v2.
Reads were mapped using STAR v2.4.2a with the reference genome hg19 for human (paremeters: --outFilterMultimapNmax 10 --outFilterMultimapScoreRange 1 --outFilterScoreMin 10).
The expression of known genes (measured in Transcripts Per Kilobase Million, TPM) was quantified using RSEM v1.2.21.
Assembly: hg19
Supplementary files format and content: Matrix table with TPM for every gene and every sample
|
| |
|
| Submission date |
Mar 10, 2022 |
| Last update date |
Oct 03, 2023 |
| Contact name |
Shibin Hu |
| E-mail(s) |
shibinhu88@gmail.com
|
| Organization name |
Stanford University
|
| Department |
Genetics
|
| Lab |
Jin Billy Li
|
| Street address |
240 pasteur drive, 4300
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL21697 |
| Series (1) |
| GSE198386 |
ADAR1 utilizes independent mechanisms to suppress MDA5 and PKR activation by cellular double-stranded RNA |
|
| Relations |
| BioSample |
SAMN26566061 |
| SRA |
SRX14432471 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
