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Sample GSM5946009 Query DataSets for GSM5946009
Status Public on Oct 03, 2023
Title HEK293T_ADAR1E921A_2
Sample type SRA
 
Source name HEK293T
Organism Homo sapiens
Characteristics cell line: HEK293T
treatment: untreated
genotype: ADAR1 RNA editing deficient
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol Reagent.
The library was constructed using KAPA HyperPrep RNA-seq Kit (Kapa Biosystems, KK8540).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 550
 
Description ADAR1_rawcount.txt
Data processing Basecalls performed using illumina RTA v2.
Reads were mapped using STAR v2.4.2a with the reference genome hg19 for human (paremeters: --outFilterMultimapNmax 10 --outFilterMultimapScoreRange 1 --outFilterScoreMin 10).
The expression of known genes (measured in Transcripts Per Kilobase Million, TPM) was quantified using RSEM v1.2.21.
Assembly: hg19
Supplementary files format and content: Matrix table with TPM for every gene and every sample
 
Submission date Mar 10, 2022
Last update date Oct 03, 2023
Contact name Shibin Hu
E-mail(s) shibinhu88@gmail.com
Organization name Stanford University
Department Genetics
Lab Jin Billy Li
Street address 240 pasteur drive, 4300
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL21697
Series (1)
GSE198386 ADAR1 utilizes independent mechanisms to suppress MDA5 and PKR activation by cellular double-stranded RNA
Relations
BioSample SAMN26566058
SRA SRX14432474

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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