GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM5946011 Query DataSets for GSM5946011
Status Public on Oct 03, 2023
Title HEK293T_ADAR1KO_2
Sample type SRA
Source name HEK293T
Organism Homo sapiens
Characteristics cell line: HEK293T
treatment: untreated
genotype: ADAR1 knockout
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol Reagent.
The library was constructed using KAPA HyperPrep RNA-seq Kit (Kapa Biosystems, KK8540).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 550
Description ADAR1_rawcount.txt
Data processing Basecalls performed using illumina RTA v2.
Reads were mapped using STAR v2.4.2a with the reference genome hg19 for human (paremeters: --outFilterMultimapNmax 10 --outFilterMultimapScoreRange 1 --outFilterScoreMin 10).
The expression of known genes (measured in Transcripts Per Kilobase Million, TPM) was quantified using RSEM v1.2.21.
Assembly: hg19
Supplementary files format and content: Matrix table with TPM for every gene and every sample
Submission date Mar 10, 2022
Last update date Oct 03, 2023
Contact name Shibin Hu
Organization name Stanford University
Department Genetics
Lab Jin Billy Li
Street address 240 pasteur drive, 4300
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL21697
Series (1)
GSE198386 ADAR1 utilizes independent mechanisms to suppress MDA5 and PKR activation by cellular double-stranded RNA
BioSample SAMN26566056
SRA SRX14432476

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap