|
| Status |
Public on Jun 07, 2022 |
| Title |
Renal cell carcinoma_C5W13 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
normal kidney cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: Jcl:SD wild-type
|
| Treatment protocol |
Male wild-type and BRCA1+/- rats at 5 weeks of age were injected intraperitonealy with Fe-NTA by the dose of 5 mg iron/kg for the first three times then 7 for the last two times for the first week, and by 7 mg iron/kg for the first time then 5 for four times for the second week, and by 7 mg iron/kg for the first three time then 10 for two times for the third week. The treatment for next 8 weeks was by the dose of 10 mg iron/kg weight, three times per week. The rats were euthanized when they were dying.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy3
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
|
| |
|
| Channel 2 |
| Source name |
RCC_C5W13
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: Jcl:SD wild-type
treatment: Fe-NTA, 3 months
|
| Treatment protocol |
Male wild-type and BRCA1+/- rats at 5 weeks of age were injected intraperitonealy with Fe-NTA by the dose of 5 mg iron/kg for the first three times then 7 for the last two times for the first week, and by 7 mg iron/kg for the first time then 5 for four times for the second week, and by 7 mg iron/kg for the first three time then 10 for two times for the third week. The treatment for next 8 weeks was by the dose of 10 mg iron/kg weight, three times per week. The rats were euthanized when they were dying.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted using DNeasy Blood & Tissue Kit (QIAGEN).
|
| Label |
Cy5
|
| Label protocol |
The extracted genomic DNA was labeled using Aagilent Sure tag DNA labeling kit according to the manufacturer's protocol (Version 8.0).
|
| |
|
| |
| Hybridization protocol |
The labeled DNA was hybridized with Agilent SurePrint G3 Mouse CGH 4x180k microarray at 67°C for 24 hours according to the manufacturer's protocol (Version 8.0).
|
| Scan protocol |
The slides were scanned in an Agilent DNA microarray scanner with SureScan High-Resolution Technology (G2565CA).
|
| Data processing |
The scanned images were analyzed with Agilent Feature Extraction Software 10.7 using default parameters (CGH_107_Sep09 protocol).
|
| |
|
| Submission date |
Mar 12, 2022 |
| Last update date |
Jun 07, 2022 |
| Contact name |
Shinya Toyokuni |
| E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
| Organization name |
Nagoya University
|
| Department |
Pathology
|
| Street address |
65 Tsuruma-Cho, Showa-Ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10451 |
| Series (1) |
| GSE198508 |
Genomic profiles of oxidative stress-induced renal cell carcinomas developed in wild-type and BRCA1+/- SD rats |
|
