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Status |
Public on Mar 25, 2022 |
Title |
20aH2 |
Sample type |
SRA |
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Source name |
Z. mobilis ZM4
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Organism |
Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821 |
Characteristics |
strain: Z. mobilis ZM4::pHW20a treatment: under the stress of 4-hydroxybenzaldehyde
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Treatment protocol |
Three replicates were carried out under the stress of 4-hydroxybenzaldehyde, syringaldehyde, and vanillin.
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Growth protocol |
Z. mobilis ZM4 was incubated in RM medium containing 20 g/L glucose, 2 g/L KH2PO4, and 10 g/L yeast extract at 30 oC without shaking .
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Extracted molecule |
polyA RNA |
Extraction protocol |
We used TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to isolate the total RNA from the freshly harvested recombinant Z. mobilis ZM4 samples. A KAPA kit (Cat no.KK4602) was used to develop a further absolute quantification of the prepared library
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Description |
20aH2_All
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Data processing |
RNA-Seq sequencing assays were carried out by CapitalBio Technology Co., Ltd, Beijing, China, using the Illumina NovaSeq 6000 platform and long-read MinION sequencer. HTSeq was used to calculate the gene count of mRNA. Supplementary files format and content: tab-delimited text files include FPKM values for each Sample. Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
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Submission date |
Mar 22, 2022 |
Last update date |
Mar 25, 2022 |
Contact name |
Xia Yi |
Organization name |
Changzhou University
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Street address |
21 Gehu Middle Road
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City |
Changzhou |
ZIP/Postal code |
213164 |
Country |
China |
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Platform ID |
GPL32076 |
Series (1) |
GSE199182 |
ZMO1162 Overexpression Boosts Phenolic Aldehyde Conversion and Ethanol Production of Zymomonas mobilis ZM4 |
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Relations |
BioSample |
SAMN26876034 |
SRA |
SRX14570261 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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