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Sample GSM5966452 Query DataSets for GSM5966452
Status Public on Mar 25, 2022
Title 20aV1
Sample type SRA
 
Source name Z. mobilis ZM4
Organism Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Characteristics strain: Z. mobilis ZM4::pHW20a
treatment: under the stress of vanillin
Treatment protocol Three replicates were carried out under the stress of 4-hydroxybenzaldehyde, syringaldehyde, and vanillin.
Growth protocol Z. mobilis ZM4 was incubated in RM medium containing 20 g/L glucose, 2 g/L KH2PO4, and 10 g/L yeast extract at 30 oC without shaking .
Extracted molecule polyA RNA
Extraction protocol We used TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to isolate the total RNA from the freshly harvested recombinant Z. mobilis ZM4 samples.
A KAPA kit (Cat no.KK4602) was used to develop a further absolute quantification of the prepared library
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description 20aV1_All
Data processing RNA-Seq sequencing assays were carried out by CapitalBio Technology Co., Ltd, Beijing, China, using the Illumina NovaSeq 6000 platform and long-read MinION sequencer.
HTSeq was used to calculate the gene count of mRNA.
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample.
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
 
Submission date Mar 22, 2022
Last update date Mar 25, 2022
Contact name Xia Yi
Organization name Changzhou University
Street address 21 Gehu Middle Road
City Changzhou
ZIP/Postal code 213164
Country China
 
Platform ID GPL32076
Series (1)
GSE199182 ZMO1162 Overexpression Boosts Phenolic Aldehyde Conversion and Ethanol Production of Zymomonas mobilis ZM4
Relations
BioSample SAMN26876029
SRA SRX14570266

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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