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Sample GSM5966457

Query DataSets for GSM5966457

Status

Public on Mar 25, 2022

Title

1162H3

Sample type

SRA

Source name

Z. mobilis ZM4

Organism

Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821

Characteristics

strain: Z. mobilis ZM4::pHW20a-ZMO1162
treatment: under the stress of 4-hydroxybenzaldehyde

Treatment protocol

Three replicates were carried out under the stress of 4-hydroxybenzaldehyde, syringaldehyde, and vanillin.

Growth protocol

Z. mobilis ZM4 was incubated in RM medium containing 20 g/L glucose, 2 g/L KH2PO4, and 10 g/L yeast extract at 30 oC without shaking .

Extracted molecule

polyA RNA

Extraction protocol

We used TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to isolate the total RNA from the freshly harvested recombinant Z. mobilis ZM4 samples.
A KAPA kit (Cat no.KK4602) was used to develop a further absolute quantification of the prepared library

Library strategy

RNA-Seq

Library source

transcriptomic

Library selection

cDNA

Instrument model

Illumina NovaSeq 6000

Description

1162H3_All

Data processing

RNA-Seq sequencing assays were carried out by CapitalBio Technology Co., Ltd, Beijing, China, using the Illumina NovaSeq 6000 platform and long-read MinION sequencer.
HTSeq was used to calculate the gene count of mRNA.
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample.
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample

Submission date

Mar 22, 2022

Last update date

Mar 25, 2022

Contact name

Xia Yi

Organization name

Changzhou University

Street address

21 Gehu Middle Road

City

Changzhou