|
| Status |
Public on Dec 21, 2022 |
| Title |
mock expressing HT-29 cells 3 |
| Sample type |
RNA |
| |
|
| Source name |
mock expressing HT-29 cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: None
cell line: HT-29
cell type: colon cancer cells
genotype: control
|
| Treatment protocol |
None
|
| Growth protocol |
HT-29 cells were cultured in 10%FBS containing RPMI media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extrated with trizol methods
|
| Label |
biotin
|
| Label protocol |
biotin-labeled with TdT (terminal deoxynucleotidyl transferase) using the GeneChip WT Terminal labeling kit
|
| |
|
| Hybridization protocol |
Approximately 5.5mg of labeled DNA target was hybridized to the Affymetrix GeneChip Array 45℃ for 16hours
|
| Scan protocol |
CGS 3000 scanner
|
| Description |
Microarray of mock expressing HT-29 cells 3
210810
|
| Data processing |
The probe cell intensity data computation and a CEL file generation was performed using Affymetrix® GeneChip Command Console® Software (AGCC).
RMA analysis
|
| |
|
| Submission date |
Apr 01, 2022 |
| Last update date |
Dec 21, 2022 |
| Contact name |
Sujin Kang |
| E-mail(s) |
sjkang3492@gmail.com
|
| Organization name |
Yonsei University
|
| Department |
Department of Systems Biology
|
| Street address |
50, Yonsei-ro, Seodaemun-gu
|
| City |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE199992 |
Microarray analysis in TRIM40-overexpressing HT-29 cells |
| GSE200024 |
Analysis of TRIM40 induced gene profiles in intestinal cells |
|
