|
| Status |
Public on Apr 09, 2022 |
| Title |
kai2-6-Heat-4 |
| Sample type |
RNA |
| |
|
| Source name |
whole seedling
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: whole seedling
genotype/variation: kai2
treatment: heat
time point: 6hr
age: 10-day old
|
| Treatment protocol |
Plant samples were divided into two groups and each group contained three trays. One group set were subjected to continuous 40°C heat stress, while control group was grown at 23°C using growth chamber
|
| Growth protocol |
10-day-old plants (45 plants from each genotype per tray) were grown on plastic trays ((21×30×5 cm in width, length and height, respectively) containing dry soil and saturated with water
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Aerial tissues of 10-day-old WT and kai2-2 mutant plants grown on soil under control and 6h and 24h heat stress conditions were detached and collected in four biological replicates for transcriptome analysis
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions,
|
| |
|
| Hybridization protocol |
1.5 ug of Cy3-labelled cRNA (specific activity >10.0 pmol Cy3/ug cRNA) was fragmented at 60°C for 30 minutes in a reaction volume of 250 ml containing 1x Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturers instructions. On completion of the fragmentation reaction, 250 ml of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized for 17 hours at 65C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 minute at room temperature with GE Wash Buffer 1 (Agilent) and 1 minute with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation.
|
| Scan protocol |
ly after washing on the Agilent DNA Microarray Scanner G2505C using one color scan setting
|
| Description |
Gene expression inkai2-6-Heat-4
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Apr 04, 2022 |
| Last update date |
Apr 10, 2022 |
| Contact name |
MOSTAFA ABDELWAHED Abdelrahman |
| E-mail(s) |
meettoo2000@yahoo.com
|
| Phone |
+201095725679
|
| Organization name |
GALALA UNNIVERSITY
|
| Street address |
Suze
|
| City |
El Sokhna |
| State/province |
SUZE |
| ZIP/Postal code |
43511 |
| Country |
Egypt |
| |
|
| Platform ID |
GPL12621 |
| Series (1) |
| GSE200100 |
Karrikin Receptor KARRIKIN INSENSITIVE2 Positively Regulates Heat Stress Tolerance in Arabidopsis thaliana |
|
