|
| Status |
Public on Sep 30, 2023 |
| Title |
The HaCaT cell lines, overexpression lentiviruses empty vectors 1 |
| Sample type |
RNA |
| |
|
| Source name |
The HaCaT cell lines were infected by overexpression lentiviruses empty vectors
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HaCaT
genotype/variation: no
|
| Treatment protocol |
The HaCaT cell lines were infected by overexpression lentiviruses vectors of GJB2 gene mutant p.Asp66His(D66H), and empty GV308 vector.
|
| Growth protocol |
The cell lines were maintained using Gibco Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin at 37°C in a humidified atmosphere containing 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 50-500ng total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16-18 hr at 45 ℃ on GeneChip PrimeView Human Gene Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 300
|
| Data processing |
The gene expression profile was preprocessed using Limma package in Bioconductor and Affymetrix annotation files. The background correction, quantile normalization and probe summarization of the microarray data were performed using the Robust Multiarray average algorithm to obtain the gene expression matrix. The cut-off for the background correction was 20%, and the coefficient of variation was 25%. The Benjamini-Hochberg method was used to correct the significant difference level (FDR).
The screening criteria for significantly different genes were: |Fold Change|>1.5 and FDR<0.05.
|
| |
|
| Submission date |
Apr 07, 2022 |
| Last update date |
Sep 30, 2023 |
| Contact name |
Li Zhang |
| E-mail(s) |
zhangliwenzhe@medmail.com.cn
|
| Organization name |
Department of Dermatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan
|
| Street address |
Department of Dermatology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250021, China.
|
| City |
Jinan |
| ZIP/Postal code |
250021 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (2) |
| GSE200386 |
Gene expression and functional analysis of GJB2 mutated HaCaT cell lines [D66H] |
| GSE200387 |
Gene expression and functional analysis of GJB2 mutated HaCaT cell lines |
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