|
| Status |
Public on Jul 21, 2023 |
| Title |
DSRCT-2 Warm Dissociation |
| Sample type |
SRA |
| |
|
| Source name |
PDX
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PDX
cell line: DSRCT-2
cell type: DSRCT cell
group: Warm
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells or nuclei were extracted from tissue either from a warm dissociation, cold dissociation, or nucleus isolation protocol. Cells or nuclei were flow sorted to enrich for single live cells and single nuclei.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The demultiplexing, barcoded processing and gene counting were made using the Cell Ranger software (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Assembly: hg38
Supplementary files format and content: Data provided for single-cell or single nucleus RNA-Seq is the raw count matrix HDF5 file from Cell Ranger
|
| |
|
| Submission date |
Apr 09, 2022 |
| Last update date |
Jul 21, 2023 |
| Contact name |
Danh Truong |
| E-mail(s) |
dtruong4@mdanderson.org
|
| Organization name |
MD Anderson Cancer Center
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| ZIP/Postal code |
TX |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE200529 |
Dissociation Protocols used for Sarcoma Tissues Bias the Transcriptome observed in Single-cell and Single-nucleus RNA sequencing. |
|
| Relations |
| BioSample |
SAMN27489042 |
| SRA |
SRX14794991 |
