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Sample GSM6035498 Query DataSets for GSM6035498
Status Public on Jul 21, 2023
Title DSRCT-2 Cold Dissociation Replicate 2
Sample type SRA
 
Source name PDX
Organism Homo sapiens
Characteristics tissue: PDX
cell line: DSRCT-2
cell type: DSRCT cell
group: Cold
Extracted molecule total RNA
Extraction protocol Cells or nuclei were extracted from tissue either from a warm dissociation, cold dissociation, or nucleus isolation protocol. Cells or nuclei were flow sorted to enrich for single live cells and single nuclei.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description 10x Genomics
Data processing The demultiplexing, barcoded processing and gene counting were made using the Cell Ranger software (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger)
Assembly: hg38
Supplementary files format and content: Data provided for single-cell or single nucleus RNA-Seq is the raw count matrix HDF5 file from Cell Ranger
 
Submission date Apr 09, 2022
Last update date Jul 21, 2023
Contact name Danh Truong
E-mail(s) dtruong4@mdanderson.org
Organization name MD Anderson Cancer Center
Street address 1515 Holcombe Blvd
City Houston
ZIP/Postal code TX
Country USA
 
Platform ID GPL24676
Series (1)
GSE200529 Dissociation Protocols used for Sarcoma Tissues Bias the Transcriptome observed in Single-cell and Single-nucleus RNA sequencing.
Relations
BioSample SAMN27489041
SRA SRX14794992

Supplementary file Size Download File type/resource
GSM6035498_DSRCT-PPX-3-1-Cold_raw_feature_bc_matrix.h5 160.6 Mb (ftp)(http) H5
GSM6035498_DSRCT-PPX-3.1-Cold_metadata.csv.gz 146.0 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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