|
Status |
Public on Jul 21, 2023 |
Title |
DSRCT-2 Cold Dissociation Replicate 2 |
Sample type |
SRA |
|
|
Source name |
PDX
|
Organism |
Homo sapiens |
Characteristics |
tissue: PDX cell line: DSRCT-2 cell type: DSRCT cell group: Cold
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells or nuclei were extracted from tissue either from a warm dissociation, cold dissociation, or nucleus isolation protocol. Cells or nuclei were flow sorted to enrich for single live cells and single nuclei.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
10x Genomics
|
Data processing |
The demultiplexing, barcoded processing and gene counting were made using the Cell Ranger software (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger) Assembly: hg38 Supplementary files format and content: Data provided for single-cell or single nucleus RNA-Seq is the raw count matrix HDF5 file from Cell Ranger
|
|
|
Submission date |
Apr 09, 2022 |
Last update date |
Jul 21, 2023 |
Contact name |
Danh Truong |
E-mail(s) |
dtruong4@mdanderson.org
|
Organization name |
MD Anderson Cancer Center
|
Street address |
1515 Holcombe Blvd
|
City |
Houston |
ZIP/Postal code |
TX |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE200529 |
Dissociation Protocols used for Sarcoma Tissues Bias the Transcriptome observed in Single-cell and Single-nucleus RNA sequencing. |
|
Relations |
BioSample |
SAMN27489041 |
SRA |
SRX14794992 |