|
Status |
Public on Jun 09, 2023 |
Title |
Mouse # 65674 tumor-infiltrating CD8 RNA-seq |
Sample type |
SRA |
|
|
Source name |
Sorted CD8+ T cells
|
Organism |
Mus musculus |
Characteristics |
genotype: dKO Sex: M
|
Extracted molecule |
total RNA |
Extraction protocol |
Sorted cells were centrifuged and resuspended in QIAzol. After addition of chloroform (Sigma) and centrifugation, RNA was isolated from the aqueous phase using an RNEasy Micro kit (QIAGEN).
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
RNA-seq of CD8+ T cells sorted from melanoma tissue at 50 days post induction
|
Data processing |
Raw reads were quasi-mapped to the Gencode M25 (GRCm38.p6) gene set using salmon v.1.2.1 (Patro et al., 2017). Assembly: Gencode M25 (GRCm38.p6) Supplementary files format and content: fastq - raw Illumina reads; sf - table of gene expression counts and TPM generated by salmon
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|
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Submission date |
Apr 13, 2022 |
Last update date |
Jun 09, 2023 |
Contact name |
Dan Filipescu |
Organization name |
Icahn School of Medicine at Mount Sinai
|
Department |
Oncological Sciences
|
Lab |
Emily Bernstein
|
Street address |
1470 Madison Ave
|
City |
New York |
State/province |
New York |
ZIP/Postal code |
10029 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE200725 |
MacroH2A restricts melanoma progression via inhibition of chemokine expression in cancer-associated fibroblasts [RNA-seq] |
GSE200751 |
MacroH2A restricts melanoma progression via inhibition of chemokine expression in cancer-associated fibroblasts. |
|
Relations |
BioSample |
SAMN27562110 |
SRA |
SRX14841423 |