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Sample GSM6043802 Query DataSets for GSM6043802
Status Public on May 29, 2024
Title HGC27 cell, lactate, rep2
Sample type SRA
Source name HGC27
Organism Homo sapiens
Characteristics cell line: HGC27
cell type: human gastric cancer cell
treatment: clutured in glucose-free DMEM with exogenous 25mM lactate
Treatment protocol Cells were cultured in glucose-free DMEM with 10% FBS and 1% P.S. antibiotics and stimulated with lactate for 24h
Growth protocol Cells were cultured in glucose-free DMEM with 10% FBS and 1% P.S. antibiotics
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol following the standard protocol
Libraries were prepared according to Illumina's instructions
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description L1EFL081643--Lac26
Data processing Illumina Cassava1.7 software is used for basecalling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low quality sequence, the mapped to hg38 whole genome
RSEM (Version 1.3.3) was used for calculating the FPKM value.
Assembly: hg38
Supplementary files format and content: tab-delimited text files include RPKM values for each sample
Submission date Apr 14, 2022
Last update date May 29, 2024
Contact name junyi ju
Organization name Shanghai Tenth People’s Hospital
Street address 301 middle yanchang road
City shanghai
ZIP/Postal code 200072
Country China
Platform ID GPL24676
Series (1)
GSE200790 Transcriptomes analyses of gastric cancer HGC-27 cell line treated with lactate
BioSample SAMN27584068
SRA SRX14852360

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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