|
| Status |
Public on Apr 28, 2022 |
| Title |
IR_F-Bm-7d |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6J wild mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Bone marrow
treat: IR
time: 7d
|
| Treatment protocol |
Bone marrow and brain tissues of mice in each group were collected 6 h, 7 days and 14 days after irradiation
|
| Growth protocol |
Mice were divided into normal control group, drug control group, radiation control group, drug irradiation group, radiation control group. Mice in the radiation control group and drug irradiation group were given 6.5 Gy 60Co γ -ray 30 min after administration.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissues by appropriate methods and purified with NucleoSpin RNA clean-up Kit (Macherey-Nagel, Germany), quantified by spectrophotometer and inspected by formaldehyde denaturing gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
Labeling was performed by Bioassay Laboratory of CapitalBio Corporation，Beijing, following their standard operating protocol. See http:www.capitalbio.com.
|
| |
|
| Hybridization protocol |
Hybridization was performed by Bioassay Laboratory of CapitalBio Corporation，Beijing, following their standard operating protocol. See http:www.capitalbio.com.
|
| Scan protocol |
Scan was performed by Bioassay Laboratory of CapitalBio Corporation，Beijing, following their standard operating protocol. See http:www.capitalbio.com.
|
| Description |
Bone marrow samples were obtained from mice in the radiation control group 7 days after irradiation.
|
| Data processing |
After data correction, such as normalization of signal value with RMA, SAM4.0 software was used for analysis.
FDR was controlled within 5%, and differential expression genes were screened by 2 or 1.5 times standard.
|
| |
|
| Submission date |
Apr 27, 2022 |
| Last update date |
Apr 28, 2022 |
| Contact name |
Zhang Xiao Chang |
| E-mail(s) |
zhangxc@bmi.ac.cn
|
| Organization name |
Beijing Institute of Radiation Medicine
|
| Street address |
Taiping 27 Road
|
| City |
Beijing |
| ZIP/Postal code |
100039 |
| Country |
China |
| |
|
| Platform ID |
GPL10192 |
| Series (1) |
| GSE201721 |
Gene expression profiles analysis ofradiation-damaged mice brain and bonemarrow after anti-radiation drugs intervention |
|
