NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM60839 Query DataSets for GSM60839
Status Public on Jul 27, 2006
Title SAGE_Drosophila pseudoobscura_Male
Sample type SAGE
Anchor NlaIII
Tag Count 23443
Tag Length 10
 
Source name male flies
Organism Drosophila pseudoobscura
Characteristics Unmated male flies aged 3 to 7 days
Extracted molecule total RNA
 
Description The mRNA was isolated from total RNA using a biotin-labeled Tn oligo and Dynal beads. Double stranded cDNA was generated with Superscript II reverse transcriptase and DNA polymerase I. The resulting cDNA library was digested with NlaIII (anchoring enzyme). 3’ restriction fragments were recovered with Dynal beads and split into two populations. Each aliquot was ligated to one of the two annealed linker pairs. After extensive washing tags were created by digestion with BsmFI (tagging enzyme). Tags were blunted with T4 polymerase and ligated to generate ditags. Ditags were PCR amplified and digested with NlaIII. Restriction fragments were separated on a 12% polyacrylamide gel and the band containing the ditags was isolated from the gel. The purified inserts were concatemerized and ligated into pZero and transformed into EDH5? electromax cells.
 
Submission date Jun 13, 2005
Last update date Nov 19, 2008
Contact name Schlötterer Christian
E-mail(s) christian.schloetterer@vu-wien.ac.at
Phone +43-1-25077-5603
Fax +43-1-25077-5693
Organization name Veterinärmedizinische Universität Wien
Department Institut für Tierzucht und Genetik
Street address Josef Baumann Gasse 1
City Wien
ZIP/Postal code A-1210
Country Austria
 
Platform ID GPL2537
Series (1)
GSE2840 No accelerated rate of protein evolution in male biased Drosophila pseudoobscura genes

Data table header descriptions
TAG 10 bp SAGE TAG
COUNT Absolute count of the tag
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAGAT 2 85.31
AAAAAACGGA 1 42.66
AAAAACAAAA 1 42.66
AAAAACACTA 1 42.66
AAAAACCACG 1 42.66
AAAAACCCTC 1 42.66
AAAAAGTAGG 1 42.66
AAAAATAACT 1 42.66
AAAAATAGGA 1 42.66
AAAAATCAAT 1 42.66
AAAACAAGCA 1 42.66
AAAACAGATA 1 42.66
AAAACCAACA 2 85.31
AAAACCACAC 2 85.31
AAAACCGAAA 1 42.66
AAAACCTCCC 1 42.66
AAAACGCGTC 1 42.66
AAAACGGATT 1 42.66
AAAACTAATA 1 42.66
AAAACTAGCT 2 85.31

Total number of rows: 7672

Table truncated, full table size 144 Kbytes.




Supplementary data files not provided

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap