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Sample GSM610397 Query DataSets for GSM610397
Status Public on Feb 09, 2012
Title Identification of miR-145 target genes in prostate cancer, PC3
Sample type RNA
 
Channel 1
Source name miR-145 transfected in prostate cancer cell line (PC3)
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
miRNA: miR-145
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or MEM.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following the manufacturer's instructions.
Label Cy3
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
 
Channel 2
Source name control microRNA-transfected cell line (PC3)
Organism Homo sapiens
Characteristics cell line: PC3
cell type: prostate cancer
miRNA: control
Treatment protocol microRNA transfection using Lipofectamine RNiMAX (Invitrogen).
Growth protocol Normal cell culture medium RPMI 1640 or MEM.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following the manufacturer's instructions.
Label Cy5
Label protocol Agilent two-color Low RNA Input Linear Amplification Kit labeling protocol.
 
 
Hybridization protocol Agilent two-color gene expression hyb/wash protocol.
Scan protocol Microarray slides were scanned in an Agilent Technologies G2505B Microarray Scanner at 5 micron resolution. Images were quantified using Agilent Feature Extraction software version 9.5.3.
Description Differentially expressed genes by miR-145 transfection.
Data processing LOWESS-normalized, background-subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent Feature Extraction software version 9.5.3 was used.
 
Submission date Oct 18, 2010
Last update date Feb 10, 2012
Contact name Naohiko Seki
E-mail(s) naoseki@faculty.chiba-u.jp
Phone 81-43-226-2971
Fax 81-43-227-3442
Organization name Chiba University
Department School of Medicine
Lab Functional Genomics
Street address 1-8-1 Inohana,Chuo-ku
City Chiba
State/province Chiba
ZIP/Postal code 260-8670
Country Japan
 
Platform ID GPL10332
Series (1)
GSE24782 Identification of target genes of tumor suppressor microRNAs in human cancer

Data table header descriptions
ID_REF
VALUE normalized log10 ratio Cy3/Cy5
INV_VALUE normalized log10 ratio Cy5/Cy3

Data table
ID_REF VALUE INV_VALUE
1 -0.0680957 6.809566701e-002
2 0.000000000e+000 0.000000000e+000
3 0.000000000e+000 0.000000000e+000
4 0.000000000e+000 0.000000000e+000
5 0.000000000e+000 0.000000000e+000
6 0.000000000e+000 0.000000000e+000
7 0.000000000e+000 0.000000000e+000
8 0.000000000e+000 0.000000000e+000
9 0.000000000e+000 0.000000000e+000
10 0.000000000e+000 0.000000000e+000
11 0.000000000e+000 0.000000000e+000
12 0.106491 -1.064912515e-001
13 0.102411 -1.024105876e-001
14 0.0718088 -7.180880163e-002
15 -0.0343874 3.438741392e-002
16 0.0780296 -7.802956955e-002
17 0.522649 -5.226493765e-001
18 0.0554256 -5.542557254e-002
19 -0.469864 4.698639689e-001
20 -0.166568 1.665684200e-001

Total number of rows: 44495

Table truncated, full table size 1479 Kbytes.




Supplementary file Size Download File type/resource
GSM610397.txt.gz 15.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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