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Sample GSM612962 Query DataSets for GSM612962
Status Public on Jan 12, 2011
Title MO2-ers2a 8 hpf - MO-control 8 hpf
Sample type RNA
 
Channel 1
Source name MO2-ers2a embryos at 8 hours post fertilization
Organism Danio rerio
Characteristics age: 8 hours post fertilization
Treatment protocol Danio rerio ovulated oocytes were microinjected with a translation blocking morpholino for estrogen receptor-beta2 (ers2a) (10.3 ng per embryo) or with control morpholino (10.3 ng per embryo).
Growth protocol Microinjected embryos were raised in fish water at 28.5 C degrees and were fixed at 8 hpf and 48 hpf
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol 200 ng of total RNA were primed and reversed transcribed using the Agilent Quick-Amp labeling Kit protocol following manufacturer's instructions
 
Channel 2
Source name MO-control embryos at 8 hours post fertilization
Organism Danio rerio
Characteristics age: 8 hours post fertilization
Treatment protocol Danio rerio ovulated oocytes were microinjected with a translation blocking morpholino for estrogen receptor-beta2 (ers2a) (10.3 ng per embryo) or with control morpholino (10.3 ng per embryo).
Growth protocol Microinjected embryos were raised in fish water at 28.5 C degrees and were fixed at 8 hpf and 48 hpf
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol 200 ng of total RNA were primed and reversed transcribed using the Agilent Quick-Amp labeling Kit protocol following manufacturer's instructions
 
 
Hybridization protocol The samples were mixed with 25X Fragmentation Buffer and 10X Blocking Agent (Agilent Gene Expression Hybridization Kit) and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential.
Scan protocol Scanned on Agilent Technologies Scanner G2505B US22502723 - Images were quantified using Agilent Feature Extraction Software
Description Danio rerio embryos injected with a translation blocking morpholino for estrogen receptor beta2 (ers2a) compared to embryos injected with control morpholino. All the embryos are fixed at 8 hours post fertilization
Data processing Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
 
Submission date Oct 26, 2010
Last update date Jan 12, 2011
Contact name Andrea Celeghin
E-mail(s) andrea.celeghin@unipd.it
Phone +39 049 8276186
Organization name University of Padua
Department Biology
Lab Comparative Endocrinology
Street address Via Ugo Bassi 58 B
City Padua
State/province Veneto
ZIP/Postal code 35131
Country Italy
 
Platform ID GPL6457
Series (1)
GSE24934 The knockdown of the maternal estrogen receptor-β2 mRNA (ers2a) affects embryo transcript contents and larval development in zebrafish

Data table header descriptions
ID_REF
VALUE normalized log10 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
1 6.08E-02
2 0.00E+00
3 0.00E+00
4 0.00E+00
5 0.00E+00
6 0.00E+00
7 0.00E+00
8 0.00E+00
9 -8.03E-01
10 -6.60E-01
11 -4.55E-01
12 2.51E-02
13 4.96E-01
14 -2.98E-02
15 9.89E-02
16 -1.55E-01
17 0.00E+00
18 -4.21E-02
19 5.92E-02
20 0.00E+00

Total number of rows: 45220

Table truncated, full table size 664 Kbytes.




Supplementary file Size Download File type/resource
GSM612962_UniversityPadova_251916110461_S01_GE2_105_Jan09_1_1.txt.gz 464.5 Kb (ftp)(http) TXT
Processed data included within Sample table

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