|
Status |
Public on Jan 12, 2011 |
Title |
MO2-ers2a 48 hpf - MO-control 48 hpf |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
MO2-ers2a embryos at 48 hours post fertilization
|
Organism |
Danio rerio |
Characteristics |
age: 48 hours post fertilization
|
Treatment protocol |
Danio rerio ovulated oocytes were microinjected with a translation blocking morpholino for estrogen receptor-beta2 (ers2a) (10.3 ng per embryo) or with control morpholino (10.3 ng per embryo).
|
Growth protocol |
Microinjected embryos were raised in fish water at 28.5 C degrees and were fixed at 8 hpf and 48 hpf
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy5
|
Label protocol |
200 ng of total RNA were primed and reversed transcribed using the Agilent Quick-Amp labeling Kit protocol following manufacturer's instructions
|
|
|
Channel 2 |
Source name |
MO-control embryos at 48 hours post fertilization
|
Organism |
Danio rerio |
Characteristics |
age: 48 hours post fertilization
|
Treatment protocol |
Danio rerio ovulated oocytes were microinjected with a translation blocking morpholino for estrogen receptor-beta2 (ers2a) (10.3 ng per embryo) or with control morpholino (10.3 ng per embryo).
|
Growth protocol |
Microinjected embryos were raised in fish water at 28.5 C degrees and were fixed at 8 hpf and 48 hpf
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
Label |
Cy3
|
Label protocol |
200 ng of total RNA were primed and reversed transcribed using the Agilent Quick-Amp labeling Kit protocol following manufacturer's instructions
|
|
|
|
Hybridization protocol |
The samples were mixed with 25X Fragmentation Buffer and 10X Blocking Agent (Agilent Gene Expression Hybridization Kit) and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequential.
|
Scan protocol |
Scanned on Agilent Technologies Scanner G2505B US22502723 - Images were quantified using Agilent Feature Extraction Software
|
Description |
Danio rerio embryos injected with a translation blocking morpholino for estrogen receptor beta2 (ers2a) compared to embryos injected with control morpholino. All the embryos are fixed at 48 hours post fertilization
|
Data processing |
Agilent Feature Extraction Software was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Oct 26, 2010 |
Last update date |
Jan 12, 2011 |
Contact name |
Andrea Celeghin |
E-mail(s) |
andrea.celeghin@unipd.it
|
Phone |
+39 049 8276186
|
Organization name |
University of Padua
|
Department |
Biology
|
Lab |
Comparative Endocrinology
|
Street address |
Via Ugo Bassi 58 B
|
City |
Padua |
State/province |
Veneto |
ZIP/Postal code |
35131 |
Country |
Italy |
|
|
Platform ID |
GPL6457 |
Series (1) |
GSE24934 |
The knockdown of the maternal estrogen receptor-β2 mRNA (ers2a) affects embryo transcript contents and larval development in zebrafish |
|