|
| Status |
Public on Oct 27, 2010 |
| Title |
blood-cells_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
blood cells, replicate 3
|
| Organism |
Ciona intestinalis |
| Characteristics |
tissue: blood cells
|
| Treatment protocol |
Each organs from single individual were dissected and dissolved into Trizol (invitrogen).
|
| Growth protocol |
Ciona intestinalis were obtained from the National BioResource Project in Japan (Department of Zoology, Kyoto University).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using RNAeasy mini kit (QIAGEN, Valencia, CA) following the manufacturer's protocols. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy5
|
| Label protocol |
Cyanine-5 (Cy5) labeled cRNA was prepared using the Quick Amp Labeling Kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
A set of fluorescently labeled cRNA targets (5 µg for each sample) was employed in a hybridization reaction. Hybridization and washing were performed using GE Hybridization Kit and GE Wash Pack (Agilent Technologies). The protocols were according to the manufacturer's instructions.
|
| Scan protocol |
Slides were scanned immediately after washing on the GenePix 4000B DNA Microarray Scanner (Axon Instruments, Foster City, CA) using one color scan setting for 1x44k array slides.
|
| Description |
Gene exression in blood cells
|
| Data processing |
The scanned images were analyzed with GenePix Pro4.0 microarray analysis software (Axon Instruments).
|
| |
|
| Submission date |
Oct 26, 2010 |
| Last update date |
Oct 26, 2010 |
| Contact name |
Mayuko Hamada |
| E-mail(s) |
hamadam@okayama-u.ac.jp
|
| Organization name |
Okayama University
|
| Lab |
Ushimado Marine Institute
|
| Street address |
130-17, Kashino, Ushimado
|
| City |
Setouchi |
| State/province |
Okayama |
| ZIP/Postal code |
701-4303 |
| Country |
Japan |
| |
|
| Platform ID |
GPL5576 |
| Series (1) |
| GSE24941 |
Transcriptome analysis of Ciona intestinalis to identify genes expressed in the adult organs. |
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