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Sample GSM617111 Query DataSets for GSM617111
Status Public on Jan 26, 2011
Title PH1_tebuconazole treated_rep3
Sample type RNA
Source name PH1_complete medium_5 mg/l tebuconazole_12h
Organism Fusarium graminearum
Characteristics strain: PH1 (syn. NRRL31084)
Treatment protocol After 24 h, CM medium of the treatment cultures was adjusted to 5 mg/l tebuconazole and incubation of all cultures continued for further 12 h.
Growth protocol 106 conidia were inoculated into 300 ml flasks each containing 100 ml complete medium (Leslie & Summerell, The Fusarium Laboratory Manual, Blackwell Publishing) and cultivated at 23°C with shaking at 150 rpm.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from mycelia grown in vitro with the Qiagen RNeasy Plant Mini Kit following the manufacturer’s protocol. The optional on-column DNase digestion step was performed using RNase-free DNase (Qiagen).
Label Cy3
Label protocol For sample preparation and array processing the Agilent protocol “One-color microarray-based gene expression analysis (Quick Amp Labelling)” was used (, the recommended volume of control RNAs (Agilent One-Color RNA Spike-In Kit) was added to 200 ng of total RNA. Thereafter, cyanine 3´- labelled cRNA was produced, using the Agilent Quick Amp Kit (one-color) according to the manufacturer’s protocol. Labelled cRNA was purified with the RNeasy mini kit (Qiagen). Yield and Cy3 incorporation rates were analyzed using a NanoDrop ND-1000 UV-VIS spectrophotometer. In addition, size distribution of cRNAs was assessed by a BioAnalyzer 2100.
Hybridization protocol For hybridizations, 600 ng of Cy3-labelled cRNA was used. Hybridization was set up in an Agilent Microarray Hybridization Chamber (G2534A) and incubated in an Agilent Hybridization Oven (G2545A) at 65°C and 10 rpm for 17 h. Thereafter, suggested washing steps were performed.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565BA) using one color scan setting for 8x15K array slides (scan resolution: 5 µm resolution).
Description Gene expression after 12 h - treatment with 5mg/l tebuconazole
Data processing The microarray scan data were analyzed by Agilent's Feature Extraction software_v10.5 obtaining background subtracted and spatially detrended signal intensities. These 'gProcessedSignal' data were converted to gene signals by a median polish algorithm as implemented in the RMA algorithm. Gene signals were normalized between experiments by quantile normalization.
Submission date Nov 03, 2010
Last update date Jan 26, 2011
Contact name Rayko Becher
Organization name Martin-Luther-University Halle-Wittenberg, Institute of Agricultural and Nutritional Sciences
Department Department of Phytopathology and Plant Protection
Street address Betty-Heimann-Str. 3
City Halle/Saale
ZIP/Postal code 06120
Country Germany
Platform ID GPL11158
Series (1)
GSE25114 Genome-wide expression profiling of Fusarium graminearum in response to azole fungicide treatment

Data table header descriptions
VALUE Normalized gene signals

Data table
FGSG_00001 61.30
FGSG_00002 91.01
FGSG_00003 16.15
FGSG_00004 17.04
FGSG_00005 6.33
FGSG_00006 7.34
FGSG_00007 20.97
FGSG_00009 73.24
FGSG_00010 6.17
FGSG_00011 199.79
FGSG_00012 41.44
FGSG_00013 140.62
FGSG_00014 74.33
FGSG_00015 136.72
FGSG_00016 15.33
FGSG_00017 41.28
FGSG_00018 3524.21
FGSG_00019 448.49
FGSG_00020 321.94
FGSG_00021 119.93

Total number of rows: 13330

Table truncated, full table size 238 Kbytes.

Supplementary file Size Download File type/resource
GSM617111.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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