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Sample GSM618133 Query DataSets for GSM618133
Status Public on May 01, 2011
Title #2691 HUH7
Sample type RNA
Source name Huh7
Organism Homo sapiens
Characteristics cell line: Huh7
cell type: Hepatocyte
permissiveness to hcv: Non-Permissive
Growth protocol Cells were maintianed in DMEM (High Glucose) supplemented with 10% Fetal Calf Serum and 1% Pennicillin/Streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Qiagen Rneasy Mini Kit according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 5 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000.
Data processing The data were analyzed with GeneChip Operating Software, version using Affymetrix default analysis settings (MAS 5.0 algorithm) and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
Submission date Nov 05, 2010
Last update date May 01, 2011
Contact name Bray Denard
Organization name University of Texas Southwestern Medical Center
Department Molecular Genetics
Lab Jin Ye Lab
Street address 5323 Harry Hines Blvd. L5.226
City Dallas
State/province TX
ZIP/Postal code 75390
Country USA
Platform ID GPL570
Series (1)
GSE25156 Host Factors with Reduced Expression in Two HCV Permissive Cell Lines as Compared to the Non-Permissive Parent Cell Line Huh7

Data table header descriptions
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 273.104 P 0.000856509
AFFX-BioB-M_at 416.579 P 6.02111e-05
AFFX-BioB-3_at 240.093 P 0.00359458
AFFX-BioC-5_at 679.089 P 0.000195116
AFFX-BioC-3_at 918.067 P 4.42873e-05
AFFX-BioDn-5_at 2954.48 P 4.42873e-05
AFFX-BioDn-3_at 6463.73 P 0.000296708
AFFX-CreX-5_at 13697.7 P 5.16732e-05
AFFX-CreX-3_at 16446.5 P 4.42873e-05
AFFX-DapX-5_at 6.99481 A 0.455413
AFFX-DapX-M_at 20.9534 A 0.156732
AFFX-DapX-3_at 16.3765 A 0.603089
AFFX-LysX-5_at 2.31361 A 0.772364
AFFX-LysX-M_at 5.78052 A 0.749204
AFFX-LysX-3_at 7.69368 A 0.313708
AFFX-PheX-5_at 2.08231 A 0.712257
AFFX-PheX-M_at 9.79267 A 0.749204
AFFX-PheX-3_at 7.11097 A 0.686277
AFFX-ThrX-5_at 10.1849 A 0.544587
AFFX-ThrX-M_at 16.0186 A 0.313723

Total number of rows: 54675

Table truncated, full table size 1636 Kbytes.

Supplementary file Size Download File type/resource
GSM618133.CEL.gz 8.7 Mb (ftp)(http) CEL
GSM618133.CHP.gz 14.2 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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