|
| Status |
Public on Feb 01, 2011 |
| Title |
HMEC, replicate 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HMEC CD24-CD44+
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human mammary epithelial cells
cell population: CD24-CD44+
|
| Treatment protocol |
Sorted HMEC cells for CD24-CD44+ and non-CD24-CD44+ cell populations using flow cytometry.
|
| Growth protocol |
Lonza medium for HMEC cultures. HMEC cells were provided by Dr. Robert Weinberg (Whitehead institute, MIT).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using the Rneasy mini plus kit according to the Qiagen protocol.
|
| Label |
Hy3
|
| Label protocol |
According to the Exiqon protocol.
|
| |
|
| Channel 2 |
| Source name |
HMEC non-CD24-CD44+
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human mammary epithelial cells
cell population: non-CD24-CD44+
|
| Treatment protocol |
Sorted HMEC cells for CD24-CD44+ and non-CD24-CD44+ cell populations using flow cytometry.
|
| Growth protocol |
Lonza medium for HMEC cultures. HMEC cells were provided by Dr. Robert Weinberg (Whitehead institute, MIT).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using the Rneasy mini plus kit according to the Qiagen protocol.
|
| Label |
Hy5
|
| Label protocol |
According to the Exiqon protocol.
|
| |
|
| |
| Hybridization protocol |
According to the Exiqon protocol.
|
| Scan protocol |
Scanning was performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 was used to read the raw intensity of the image.
|
| Data processing |
The ratio of red signal to green signal was calculated after background subtraction and normalization using the non-linear regression algorithm.
|
| |
|
| Submission date |
Nov 08, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Mien-chie Hung |
| E-mail(s) |
mhung@mdanderson.org
|
| Phone |
7137923668
|
| Organization name |
M.D. Anderson Cancer Center
|
| Department |
Molecular and Cellular Oncology
|
| Street address |
1515 Holcomb Blvd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL11181 |
| Series (2) |
| GSE25036 |
miRNA expression profiling in human mammary epithelial cell (HMEC) CD24-CD44+ and non-CD24-CD44+ cell populations |
| GSE25037 |
p53 regulates epithelial-mesenchymal transition (EMT) and stem cell properties through modulation of miRNAs. |
|
