|
Status |
Public on Nov 10, 2010 |
Title |
JARID1B MCF-7 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
enriched DNA using anti-JARID1B
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 breast cancer cell line antibody: JARID1B antibody vendor: santa cruz antibody lot/bach#: A0108 antibody catalog#: sc-67035
|
Extracted molecule |
genomic DNA |
Extraction protocol |
common cell lysate
|
Label |
Cy5
|
Label protocol |
primer labeled with fluorescence molecules, protocol for labelling the extract follows the "Complete Product Handbook for The Aviva Systems Biology ChIP-GLAS System" (in attachment), IX. Detailed Protocol, Step 5. PCR Amplification and Labeling of GLAS Products
|
|
|
Channel 2 |
Source name |
Input DNA
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF-7 breast cancer cell line
|
Extracted molecule |
genomic DNA |
Extraction protocol |
common cell lysate
|
Label |
Cy3
|
Label protocol |
primer labeled with fluorescence molecules, protocol for labelling the extract follows the "Complete Product Handbook for The Aviva Systems Biology ChIP-GLAS System" (in attachment), IX. Detailed Protocol, Step 5. PCR Amplification and Labeling of GLAS Products
|
|
|
|
Hybridization protocol |
standard AVIVA method
|
Scan protocol |
CapitalBio manual
|
Description |
whole genome target analysis for JARID1B
|
Data processing |
After background correction and removal of bad spots, expression ratio was linear normalized using 50 percentile rank software: R version 2.10.1 (2009-12-14) Copyright (C) 2009 The R Foundation for Statistical Computing ISBN 3-900051-07-0
|
|
|
Submission date |
Nov 09, 2010 |
Last update date |
Nov 10, 2010 |
Contact name |
Shang Yongfeng |
E-mail(s) |
yshang@hsc.pku.edu.cn
|
Phone |
86-10-82805118
|
Fax |
86-10-82801355
|
Organization name |
Peking University
|
Department |
Biochemistry and Molecular Biology
|
Street address |
Hai dian district,38# Xue Yuan Road
|
City |
Beijing |
ZIP/Postal code |
100191 |
Country |
China |
|
|
Platform ID |
GPL7765 |
Series (1) |
GSE25214 |
Profiles of JARID1B target genes in MCF-7 cell line |
|