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Sample GSM620794 Query DataSets for GSM620794
Status Public on Nov 11, 2010
Title Kidney cortical Thick Ascending Limb of Henle's Loop (CTAL)
Sample type SAGE
Anchor Sau3A
Tag Count 87760
Tag Length 15
 
Source name microdissected cortical thick ascending limb of Henle's loop (CTAL) from kidney
Organism Mus musculus
Characteristics strain: CD1
gender: male
age: 10 weeks
tissue: kidney cortical thick ascending limb of Henle's loop (CTAL)
Extracted molecule polyA RNA
Extraction protocol Dynabeads mRNA direct kit (Dynal) was used to extract mRNA.
 
Description Library was obtained from 1000 microdissected cortical thick ascending limb of Henle's loop from male CD1 mice under control condition. The library was generated using Sau3AI as anchoring enzyme and MmeI as tagging enzyme.The SAGE Adaptation method for Downsized Extracts was used to generate the library. Data were provided after curation as described in the publication.
Data processing Automatic tag detection and counting was performed with SADELAB (CEA Saclay, France), a web-based integrated platform dedicated to the management of SAGE project.
 
Submission date Nov 09, 2010
Last update date Nov 10, 2010
Contact name Lydie Cheval
E-mail(s) lydie.cheval@crc.jussieu.fr
Phone (33) 1 55 42 78 60
Fax (33) 1 46 33 41 72
Organization name Institut des Cordeliers
Lab UMR 872
Street address 15 rue de l'├ęcole de M├ędecine
City Paris
ZIP/Postal code 75270
Country France
 
Platform ID GPL5119
Series (1)
GSE25223 Atlas of gene expression in the mouse kidney

Data table header descriptions
TAG
COUNT
TPM tags per million

Data table
TAG COUNT TPM
TTTATAATTTGAGAG 5030 57315.41
AACAAATCTCCTAGG 4251 48438.92
AACTTATACATCTTC 2974 33887.88
TGCTTCAATAATTTA 1871 21319.51
AAACTTTACCATTAT 1489 16966.73
GCTTTCAGCAATGGA 801 9127.16
TAAGATGAGACACAC 672 7657.25
TTGTGTCAGTCAGGT 646 7360.98
GCCTGGAGAAATGAC 622 7087.51
AACATTCCTTATTGT 574 6540.57
CACCAAACCCTAAAA 466 5309.94
GTTCTCACCCCGCAG 466 5309.94
AAACAACCCAACAGG 446 5082.04
TGCCTGTCACTGTGG 381 4341.39
TTGATATTTGGCCTC 379 4318.60
TAACCATAGCTTTAT 346 3942.57
ACAAAGTTTGAGAAT 330 3760.26
TTCCCAGAAACCAAG 314 3577.94
TGCCGCAAGTGCTAC 313 3566.55
CATGGGAGAGCCCAG 299 3407.02

Total number of rows: 17889

Table truncated, full table size 421 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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