|
| Status |
Public on Feb 01, 2011 |
| Title |
shp53, replicate 1 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
MCF12A, shp53
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF12A
retroviral expression: shp53
|
| Treatment protocol |
Retroviral infection with retrovirues expressing shp53 or shVector.
|
| Growth protocol |
Standard growth medium for the MCF12A cell line.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using the Rneasy mini plus kit according to the Qiagen protocol.
|
| Label |
Hy3
|
| Label protocol |
According to the Exiqon protocol.
|
| |
|
| Channel 2 |
| Source name |
MCF12A, shVector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF12A
retroviral expression: shVector
|
| Treatment protocol |
Retroviral infection with retrovirues expressing shp53 or shVector.
|
| Growth protocol |
Standard growth medium for the MCF12A cell line.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA using the Rneasy mini plus kit according to the Qiagen protocol.
|
| Label |
Hy5
|
| Label protocol |
According to the Exiqon protocol.
|
| |
|
| |
| Hybridization protocol |
According to the Exiqon protocol.
|
| Scan protocol |
Scanning was performed with the Axon GenePix 4000B microarray scanner. GenePix pro V6.0 was used to read the raw intensity of the image.
|
| Data processing |
The ratio of red signal to green signal was calculated after background subtraction and normalization using the non-linear regression algorithm.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Feb 01, 2011 |
| Contact name |
Mien-chie Hung |
| E-mail(s) |
mhung@mdanderson.org
|
| Phone |
7137923668
|
| Organization name |
M.D. Anderson Cancer Center
|
| Department |
Molecular and Cellular Oncology
|
| Street address |
1515 Holcomb Blvd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL11181 |
| Series (2) |
| GSE25035 |
The role of p53 in the regulation of miRNA expression profiling |
| GSE25037 |
p53 regulates epithelial-mesenchymal transition (EMT) and stem cell properties through modulation of miRNAs. |
|
