|
| Status |
Public on Nov 11, 2010 |
| Title |
Kidney Connecting Tubule (CNT) |
| Sample type |
SAGE |
| Anchor |
Sau3A
|
| Tag Count |
68600 |
| Tag Length |
15 |
| |
|
| Source name |
microdissected connecting tubule from kidney
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1
gender: male
age: 10 weeks
tissue: kidney connecting tubule
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Dynabeads mRNA direct kit (Dynal) was used to extract mRNA.
|
| |
|
| Description |
Library was obtained from 1000 microdissected Connecting Tubule from male CD1 mice under control condition. The library was generated using Sau3AI as anchoring enzyme and MmeI as tagging enzyme.The SAGE Adaptation method for Downsized Extracts was used to generate the library. Data were provided after curation as described in the publication.
|
| Data processing |
Automatic tag detection and counting was performed with SADELAB (CEA Saclay, France), a web-based integrated platform dedicated to the management of SAGE project.
|
| |
|
| Submission date |
Nov 09, 2010 |
| Last update date |
Nov 10, 2010 |
| Contact name |
Lydie Cheval |
| E-mail(s) |
lydie.cheval@crc.jussieu.fr
|
| Phone |
(33) 1 55 42 78 60
|
| Fax |
(33) 1 46 33 41 72
|
| Organization name |
Institut des Cordeliers
|
| Lab |
UMR 872
|
| Street address |
15 rue de l'école de Médecine
|
| City |
Paris |
| ZIP/Postal code |
75270 |
| Country |
France |
| |
|
| Platform ID |
GPL5119 |
| Series (1) |
| GSE25223 |
Atlas of gene expression in the mouse kidney |
|
