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Sample GSM6209500 Query DataSets for GSM6209500
Status Public on Aug 17, 2022
Title CTH-19
Sample type RNA
 
Source name Unsorted Bone Marrow Cells
Organism Homo sapiens
Characteristics disease state: T-ALL
cell type: Unsorted Bone Marrow Cells
chromothripsis status: negative
phenotype: immature
Extracted molecule total RNA
Extraction protocol RNA was extracted from bone marrow or peripheral blood samples using a QIAamp RNA Blood Mini Kit (Qiagen) at a QIAcube automated station
Label biotin
Label protocol GeneChip® WT PLUS Reagent Kit (Affymetrix Applied Biosystem Microarray, Thermo Fisher, Milan, Italy) was used to generates amplified and biotinylated sense-strand DNA targets from total RNA; it uses a reverse transcription priming method that primes the entire length of RNA. Amplified cDNA was quantified, fragmented, marked, and prepared for hybridization from the GeneChip® Clariom S Human Array (Thermo Fisher Scientific) for expression level measurement
 
Hybridization protocol Hybridization of the microarrays was performed in a GeneChip® Hybridization Oven 645 (Affymetrix/Thermo Fisher Scientific). GeneChips were washed and stained
Scan protocol Arrays were scanned using the GeneChip Scanner 3000 (Affymetrix Applied Biosystem Microarray, Thermo Fisher, Milan, Italy)
Data processing Primary data analysis was performed with Transcriptome Analysis Console software version 4.0 (TAC 4.0.2.15). Differentially expressed RNA were estimated by fold-change filtering combined with Student’s t-test. Transcripts with fold change in expression ≥ ±2.0 and FDR<0.05 were considered significantly differentially expressed. Hierarchical clustering was performed to show the distinguishing genes expression pattern among samples
 
Submission date Jun 01, 2022
Last update date Aug 17, 2022
Contact name Cristina Mecucci
E-mail(s) cristina.mecucci@unipg.it
Phone +39 075 5783808
Organization name University of Perugia
Department Department of Medicine, Hematology and Bone Marrow Transplantation Unit
Lab Laboratory of Cytogenetics and Molecular Genetics
Street address Piazzale Menghini n.9
City Perugia
ZIP/Postal code 06132
Country Italy
 
Platform ID GPL23159
Series (2)
GSE205265 Chromothripsis is a frequent event and underlies typical genetic changes in early T-cell precursor lymphoblastic leukemia in adults [gene expression]
GSE205270 Chromothripsis is a frequent event and underlies typical genetic changes in early T-cell precursor lymphoblastic leukemia in adults

Data table header descriptions
ID_REF
VALUE RMA
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
AFFX-BkGr-GC03_st 7.00126 0.751337
AFFX-BkGr-GC04_st 7.12153 0.838773
AFFX-BkGr-GC05_st 7.04621 0.901244
AFFX-BkGr-GC06_st 6.82342 0.905416
AFFX-BkGr-GC07_st 6.43388 0.897549
AFFX-BkGr-GC08_st 4.32448 0.885221
AFFX-BkGr-GC09_st 3.7748 0.867592
AFFX-BkGr-GC10_st 3.54922 0.840276
AFFX-BkGr-GC11_st 3.31791 0.840547
AFFX-BkGr-GC12_st 3.17724 0.760766
AFFX-BkGr-GC13_st 3.03186 0.777196
AFFX-BkGr-GC14_st 2.93986 0.773449
AFFX-BkGr-GC15_st 2.89388 0.767704
AFFX-BkGr-GC16_st 3.11053 0.740687
AFFX-BkGr-GC17_st 3.1773 0.719717
AFFX-BkGr-GC18_st 3.55579 0.676481
AFFX-BkGr-GC19_st 6.65097 0.590761
AFFX-BkGr-GC20_st 6.65238 0.584631
AFFX-BkGr-GC21_st 6.77613 0.581303
AFFX-BkGr-GC22_st 6.83084 0.582429

Total number of rows: 24351

Table truncated, full table size 900 Kbytes.




Supplementary file Size Download File type/resource
GSM6209500_CTH-19_Clariom_S_Human_.CEL.gz 1.2 Mb (ftp)(http) CEL
GSM6209500_CTH-19_Clariom_S_Human_.sst-rma-gene-full.chp.gz 270.9 Kb (ftp)(http) CHP
Processed data included within Sample table

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