|
| Status |
Public on Feb 08, 2023 |
| Title |
HT_3d_IP_Rep2 |
| Sample type |
SRA |
| |
|
| Source name |
HUVEC
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
cell type: Human umbilical vein endothelial cells
chip antibody: H3K27ac (Active motif #39133)
|
| Treatment protocol |
HUVECs were cultured in complete medium. For normal glucose, 25 mM mannitol was added. For high glucose, cells were treated with 25 mM glucose and 5 ng/ml TNFa. Cells were harvested after 3 and 7 days.
|
| Growth protocol |
HUVECs were grown following the manufacturer's protocol
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor (Diagenode).
The libraries were constructed using the KAPA HyperPrep kit following the user manual.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
none provided by the submitter
Assembly: hg38
|
| |
|
| Submission date |
Jun 01, 2022 |
| Last update date |
Feb 08, 2023 |
| Contact name |
Zhen Chen |
| E-mail(s) |
zhenchenlab@gmail.com
|
| Organization name |
City of Hope
|
| Department |
DMRI
|
| Street address |
1500 E Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL20795 |
| Series (2) |
| GSE205277 |
Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIP-Seq) |
| GSE205287 |
Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery |
|
| Relations |
| BioSample |
SAMN28813338 |
| SRA |
SRX15557353 |
