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Sample GSM6255112 Query DataSets for GSM6255112
Status Public on Oct 05, 2023
Title WD WT1
Sample type SRA
 
Source name Macrophage
Organism Mus musculus
Characteristics tissue: Macrophage
cell type: Macrophage
genotype: WT
treatment: Western Diet
Extracted molecule total RNA
Extraction protocol Whole aortas were harvested and separated from perivascular adipose tissue, then digested for 40 minutes at 37ÂșC in RPMI containing 450 U/ml Collagenase I, 125 U/ml Collagenase XI and 60 U/ml Hyaluronidase. Viable CD45+ and F4/80+ cells were sorted in a FACS Aria II and recovered in lysis buffer.
cDNA was harvested using SMART-Seq v4 Ultra Low Input RNA Kit following manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description AR9897
Data processing RNA-seq reads mapped using STAR software v2.5.3a with ENCODE parameters.
Annotated genes quantified by RSEM v1.3.0
Annotation version gencode M25
Assembly: GRCm38
Supplementary files format and content: Matrix with samples as columns and raw gene counts as rows
 
Submission date Jun 20, 2022
Last update date Oct 05, 2023
Contact name Antonio Postigo
Organization name IDIBAPS/ICREA
Lab Group of Regulation of Gene Expression in Cell Differentiation and Cancer
Street address Casanova 143. Cellex 1B
City Barcelona
State/province BARCELONA
ZIP/Postal code 08036
Country Spain
 
Platform ID GPL21103
Series (1)
GSE206477 Gene expression in macrophages from mice fed in Western diet
Relations
BioSample SAMN29208618
SRA SRX15798932

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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