|
| Status |
Public on Jun 07, 2023 |
| Title |
HAS_Injected-6 |
| Sample type |
SRA |
| |
|
| Source name |
Jeunum
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: Jeunum
genetic line: HAS
treatment: SRBC injected
days post_injection: 5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from each jejunum sample using the RNEasy mini kit (Qiagen, Hilden, Germany) following manufacturer protocol
Illumina RNA libraries were constructed following manufacturer protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
CLC Genomics Workbench v11 was used for all sequence processing
Adapters were trimmed and sequences were checked for quality during import into CLC Genomics Workbench v11
Sequences for each sample were mapped to the Galgal6 reference genome (GCA_000002315.5) and counts for each transcript were determined in transcripts per million using the RNA-Seq Analysis tool in CLC Genomics Workbench v11
Assembly: Galgal6: GCA_000002315.5
Supplementary files format and content: text tab delimited files including TPM for each sample
|
| |
|
| Submission date |
Jun 23, 2022 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Shelly Jones Nolin |
| E-mail(s) |
snjones@ncsu.edu
|
| Phone |
9195155537
|
| Organization name |
NC State University
|
| Department |
Prestage Dept of Poultry Science
|
| Lab |
Poultry Genomics
|
| Street address |
2711 Founders Dr
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL19005 |
| Series (1) |
| GSE206804 |
Comparative RNA sequencing of the jejuna of two lines of chickens divergently selected for antibody response to sheep red blood cells, with and without antigen exposure |
|
| Relations |
| BioSample |
SAMN29267177 |
| SRA |
SRX15849302 |
