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Sample GSM6263901 Query DataSets for GSM6263901
Status Public on Jun 07, 2023
Title LAS_Not-injected-5
Sample type SRA
 
Source name Jeunum
Organism Gallus gallus
Characteristics tissue: Jeunum
genetic line: LAS
treatment: None
days post_injection: 5
Extracted molecule total RNA
Extraction protocol RNA was isolated from each jejunum sample using the RNEasy mini kit (Qiagen, Hilden, Germany) following manufacturer protocol
Illumina RNA libraries were constructed following manufacturer protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing CLC Genomics Workbench v11 was used for all sequence processing
Adapters were trimmed and sequences were checked for quality during import into CLC Genomics Workbench v11
Sequences for each sample were mapped to the Galgal6 reference genome (GCA_000002315.5) and counts for each transcript were determined in transcripts per million using the RNA-Seq Analysis tool in CLC Genomics Workbench v11
Assembly: Galgal6: GCA_000002315.5
Supplementary files format and content: text tab delimited files including TPM for each sample
 
Submission date Jun 23, 2022
Last update date Jun 07, 2023
Contact name Shelly Jones Nolin
E-mail(s) snjones@ncsu.edu
Phone 9195155537
Organization name NC State University
Department Prestage Dept of Poultry Science
Lab Poultry Genomics
Street address 2711 Founders Dr
City Raleigh
State/province NC
ZIP/Postal code 27695
Country USA
 
Platform ID GPL19005
Series (1)
GSE206804 Comparative RNA sequencing of the jejuna of two lines of chickens divergently selected for antibody response to sheep red blood cells, with and without antigen exposure
Relations
BioSample SAMN29267160
SRA SRX15849319

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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