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Sample GSM62801 Query DataSets for GSM62801
Status Public on Aug 26, 2005
Title Mouse_MF1_E11.5_necortex_rostral_vs_caudal_r1
Sample type RNA
Channel 1
Source name rostral_pool1
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name caudal_pool1
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: rostral or caudal thirds of E11.5 MF1 neocortex where pooled from three embryos. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filted to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel where less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2854 Comparison of Rostral and Caudal murine neocortex at E11.5

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 -0.246
CG_Mm_EXT_3110714_1 0.018
CG_MmCTRL_30001_02 -0.543
CG_MmCTRL_30001_03 -0.709
CG_MmCTRL_30001_04 -0.164
CG_MmCTRL_30001_05 -0.218
CG_MmCTRL_30001_06 -0.61
CG_MmCTRL_30001_07 -0.594
CG_MmCTRL_30001_08 0.253
CG_MmCTRL_30001_09 -0.364
CG_MmCTRL_30001_10 -0.509
CG_MmCTRL_30001_100 -0.07
CG_MmCTRL_30001_101 -0.104
CG_MmCTRL_30001_102 -0.032
CG_MmCTRL_30001_103 -0.165
CG_MmCTRL_30001_104 -0.158
CG_MmCTRL_30001_105 -0.177
CG_MmCTRL_30001_106 -0.076
CG_MmCTRL_30001_107 -0.094
CG_MmCTRL_30001_108 -0.071

Total number of rows: 21998

Table truncated, full table size 530 Kbytes.

Supplementary data files not provided

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