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Sample GSM62805 Query DataSets for GSM62805
Status Public on Aug 26, 2005
Title Mouse_MF1_E11.5_necortex_rostral_vs_caudal_r3
Sample type RNA
Channel 1
Source name rostral_pool3
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name caudal_pool3
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: rostral or caudal thirds of E11.5 MF1 neocortex where pooled from three embryos. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filted to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel where less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2854 Comparison of Rostral and Caudal murine neocortex at E11.5

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 -0.153
CG_Mm_EXT_3110714_1 0.214
CG_MmCTRL_30001_02 -0.235
CG_MmCTRL_30001_03 -0.5
CG_MmCTRL_30001_04 -0.097
CG_MmCTRL_30001_05 -0.207
CG_MmCTRL_30001_06 -0.329
CG_MmCTRL_30001_07 -0.479
CG_MmCTRL_30001_08 0.17
CG_MmCTRL_30001_09 -0.295
CG_MmCTRL_30001_10 -0.407
CG_MmCTRL_30001_100 0.07
CG_MmCTRL_30001_101 0.148
CG_MmCTRL_30001_102 0.019
CG_MmCTRL_30001_103 0.043
CG_MmCTRL_30001_104 0.09
CG_MmCTRL_30001_105 -0.145
CG_MmCTRL_30001_106 0.043
CG_MmCTRL_30001_107 0.029
CG_MmCTRL_30001_108 0.023

Total number of rows: 21998

Table truncated, full table size 524 Kbytes.

Supplementary data files not provided

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