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Sample GSM62812 Query DataSets for GSM62812
Status Public on Aug 26, 2005
Title Mouse_MF1_E13.5_necortex_rostral_vs_caudal_r3_dyeswap
Sample type RNA
Channel 1
Source name caudal_pool3
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name rostral_pool3
Organism Mus musculus
Characteristics see description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: rostral or caudal thirds of E13.5 MF1 neocortex where pooled from three embryos. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2855 Comparison of Rostral and Caudal murine neocortex at E13.5

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 0.471
CG_Mm_EXT_3110714_1 0.35
CG_MmCTRL_30001_02 0.238
CG_MmCTRL_30001_03 0.033
CG_MmCTRL_30001_04 0.126
CG_MmCTRL_30001_05 0.218
CG_MmCTRL_30001_06 -0.05
CG_MmCTRL_30001_07 0.116
CG_MmCTRL_30001_08 0.181
CG_MmCTRL_30001_09 0.195
CG_MmCTRL_30001_10 0.023
CG_MmCTRL_30001_100 0.182
CG_MmCTRL_30001_101 0.135
CG_MmCTRL_30001_102 0.248
CG_MmCTRL_30001_103 0.043
CG_MmCTRL_30001_104 0.219
CG_MmCTRL_30001_105 0.165
CG_MmCTRL_30001_106 0.158
CG_MmCTRL_30001_107 0.215
CG_MmCTRL_30001_108 0.164

Total number of rows: 21998

Table truncated, full table size 538 Kbytes.

Supplementary data files not provided

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