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Sample GSM62822 Query DataSets for GSM62822
Status Public on Aug 26, 2005
Title Mouse_E13.5_Front_Middle_Back_4
Sample type RNA
Channel 1
Source name R1
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name C2
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: Three pools of rostral (R1-3), three pools of middle (M1-3), and three pools of caudal (C1-3) E13.5 MF1 neocortex were collected from 4 embryos. All possible 18 comparisons were carried out. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2856 Comparison of Rostral, Middle, and Caudal E13.5 murine neocortex

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 -0.021
CG_Mm_EXT_3110714_1 0.212
CG_MmCTRL_30001_02 0.349
CG_MmCTRL_30001_03 0.403
CG_MmCTRL_30001_04 0.416
CG_MmCTRL_30001_05 0.391
CG_MmCTRL_30001_06 0.398
CG_MmCTRL_30001_07 0.477
CG_MmCTRL_30001_08 0.427
CG_MmCTRL_30001_09 0.426
CG_MmCTRL_30001_10 0.366
CG_MmCTRL_30001_100 0.478
CG_MmCTRL_30001_101 0.456
CG_MmCTRL_30001_102 0.459
CG_MmCTRL_30001_103 0.471
CG_MmCTRL_30001_104 0.489
CG_MmCTRL_30001_105 0.457
CG_MmCTRL_30001_106 0.488
CG_MmCTRL_30001_107 0.469
CG_MmCTRL_30001_108 0.477

Total number of rows: 21998

Table truncated, full table size 526 Kbytes.

Supplementary data files not provided

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