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Sample GSM62831 Query DataSets for GSM62831
Status Public on Aug 26, 2005
Title Mouse_E13.5_Front_Middle_Back_13
Sample type RNA
 
Channel 1
Source name R1
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name M2
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: Three pools of rostral (R1-3), three pools of middle (M1-3), and three pools of caudal (C1-3) E13.5 MF1 neocortex were collected from 4 embryos. All possible 18 comparisons were carried out. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
 
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
E-mail(s) stephen.sansom@kennedy.ox.ac.uk
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE2856 Comparison of Rostral, Middle, and Caudal E13.5 murine neocortex

Data table header descriptions
ID_REF
VALUE Log ratio, lowess normalised, background filtered

Data table
ID_REF VALUE
CG_Mm_3001006_1 0.212
CG_Mm_EXT_3110714_1 0.234
CG_MmCTRL_30001_02 0.529
CG_MmCTRL_30001_03 0.31
CG_MmCTRL_30001_04 0.417
CG_MmCTRL_30001_05 0.468
CG_MmCTRL_30001_06 0.426
CG_MmCTRL_30001_07 0.394
CG_MmCTRL_30001_08 0.489
CG_MmCTRL_30001_09 0.433
CG_MmCTRL_30001_10 0.449
CG_MmCTRL_30001_100 0.428
CG_MmCTRL_30001_101 0.543
CG_MmCTRL_30001_102 0.497
CG_MmCTRL_30001_103 0.468
CG_MmCTRL_30001_104 0.537
CG_MmCTRL_30001_105 0.451
CG_MmCTRL_30001_106 0.466
CG_MmCTRL_30001_107 0.528
CG_MmCTRL_30001_108 0.444

Total number of rows: 21998

Table truncated, full table size 525 Kbytes.




Supplementary data files not provided

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