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Sample GSM62834 Query DataSets for GSM62834
Status Public on Aug 26, 2005
Title Mouse_E13.5_Front_Middle_Back_16
Sample type RNA
Channel 1
Source name M1
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name C2
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: Three pools of rostral (R1-3), three pools of middle (M1-3), and three pools of caudal (C1-3) E13.5 MF1 neocortex were collected from 4 embryos. All possible 18 comparisons were carried out. RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2856 Comparison of Rostral, Middle, and Caudal E13.5 murine neocortex

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 -0.086
CG_Mm_EXT_3110714_1 0.105
CG_MmCTRL_30001_02 0.097
CG_MmCTRL_30001_03 0.017
CG_MmCTRL_30001_04 0.134
CG_MmCTRL_30001_05 0.082
CG_MmCTRL_30001_06 0.084
CG_MmCTRL_30001_07 0.132
CG_MmCTRL_30001_08 0.278
CG_MmCTRL_30001_09 0.122
CG_MmCTRL_30001_10 0.125
CG_MmCTRL_30001_100 0.199
CG_MmCTRL_30001_101 0.253
CG_MmCTRL_30001_102 0.247
CG_MmCTRL_30001_103 0.228
CG_MmCTRL_30001_104 0.246
CG_MmCTRL_30001_105 0.176
CG_MmCTRL_30001_106 0.288
CG_MmCTRL_30001_107 0.137
CG_MmCTRL_30001_108 0.188

Total number of rows: 21998

Table truncated, full table size 526 Kbytes.

Supplementary data files not provided

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