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Sample GSM62839 Query DataSets for GSM62839
Status Public on Aug 26, 2005
Title Mouse_Peg1_E12.5_mutant_vs_wildtype_3
Sample type RNA
Channel 1
Source name WT9
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name M6
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: single E12.5 Peg1 mutant cortices were directely compared to single E12.5 wild type cortices. Six mutant cortices (M1,3,4,5,6,7) were compared to three wild type cortices (WT2,8,9). As Mest is an imprinted gene that is only expressed from the paternal allele, mutants were generated by crossing males carrying a LacZ-targeted allele in which exons 3-8 were removed to wild-type females, as described (Lefebvre, L., Viville, S., Barton, S. C., Ishino, F., Keverne, E. B. and Surani, M. A. (1998). Abnormal maternal behaviour and growth retardation associated with loss of the imprinted gene Mest. Nat Genet 20, 163-9.) RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2858 Comparison of gene expression in Peg1 wild type and null murine telencephalons

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 0.421
CG_Mm_EXT_3110714_1 0.269
CG_MmCTRL_30001_02 -0.018
CG_MmCTRL_30001_03 0.112
CG_MmCTRL_30001_04 0.121
CG_MmCTRL_30001_05 0.037
CG_MmCTRL_30001_06 0.112
CG_MmCTRL_30001_07 0.084
CG_MmCTRL_30001_08 0.194
CG_MmCTRL_30001_09 0.06
CG_MmCTRL_30001_10 0.086
CG_MmCTRL_30001_100 0.231
CG_MmCTRL_30001_101 0.141
CG_MmCTRL_30001_102 0.11
CG_MmCTRL_30001_103 0.153
CG_MmCTRL_30001_104 0.161
CG_MmCTRL_30001_105 0.13
CG_MmCTRL_30001_106 0.189
CG_MmCTRL_30001_107 0.127
CG_MmCTRL_30001_108 0.202

Total number of rows: 21998

Table truncated, full table size 529 Kbytes.

Supplementary data files not provided

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