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Sample GSM62842 Query DataSets for GSM62842
Status Public on Aug 26, 2005
Title Mouse_Peg1_E12.5_mutant_vs_wildtype_6
Sample type RNA
Channel 1
Source name M7
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
Channel 2
Source name WT9
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: single E12.5 Peg1 mutant cortices were directely compared to single E12.5 wild type cortices. Six mutant cortices (M1,3,4,5,6,7) were compared to three wild type cortices (WT2,8,9). As Mest is an imprinted gene that is only expressed from the paternal allele, mutants were generated by crossing males carrying a LacZ-targeted allele in which exons 3-8 were removed to wild-type females, as described (Lefebvre, L., Viville, S., Barton, S. C., Ishino, F., Keverne, E. B. and Surani, M. A. (1998). Abnormal maternal behaviour and growth retardation associated with loss of the imprinted gene Mest. Nat Genet 20, 163-9.) RNA was extracted by the Invitrogen Trizol Protocol, Ref: Chomczynski, P. and Sacchi, N. (1987) Analytical Biochemistry 162, 156.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
Platform ID GPL2584
Series (1)
GSE2858 Comparison of gene expression in Peg1 wild type and null murine telencephalons

Data table header descriptions
VALUE Log ratio, lowess normalised, background filtered

Data table
CG_Mm_3001006_1 0.007
CG_Mm_EXT_3110714_1 0.542
CG_MmCTRL_30001_02 0.313
CG_MmCTRL_30001_03 0.466
CG_MmCTRL_30001_04 0.417
CG_MmCTRL_30001_05 0.312
CG_MmCTRL_30001_06 0.491
CG_MmCTRL_30001_07 0.452
CG_MmCTRL_30001_08 0.474
CG_MmCTRL_30001_09 0.47
CG_MmCTRL_30001_10 0.421
CG_MmCTRL_30001_100 0.601
CG_MmCTRL_30001_101 0.522
CG_MmCTRL_30001_102 0.423
CG_MmCTRL_30001_103 0.536
CG_MmCTRL_30001_104 0.484
CG_MmCTRL_30001_105 0.52
CG_MmCTRL_30001_106 0.609
CG_MmCTRL_30001_107 0.561
CG_MmCTRL_30001_108 0.562

Total number of rows: 21998

Table truncated, full table size 527 Kbytes.

Supplementary data files not provided

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