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Sample GSM62848 Query DataSets for GSM62848
Status Public on Aug 26, 2005
Title Mouse_FGFR1_E12.5_hets_vs_hom_6
Sample type RNA
 
Channel 1
Source name HET8
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name HOM7
Organism Mus musculus
Characteristics See description
Extracted molecule total RNA
Label Cy5
 
 
Hybridization protocol See Livesey, F. J., Furukawa, T., Steffen, M. A., Church, G. M. and Cepko, C. L. (2000). Microarray analysis of the transcriptional network controlled by the photoreceptor homeobox gene Crx. Curr Biol 10, 301-10
Scan protocol Hybridised microarrays were scanned on a GenePix 4000B microarray scanner and the resulting images were analysed with GenePix 5 array analysis software.
Description Sample details: We analysed changes in gene expression in the dorsal telencephalon of forebrain-specific FGFR1 mutant mice. Gene expression was compared between the dorsal telencephalons of single E12.5 embryos of two genotypes: FGFR1 heterozygous (HET1,3,8) and homozygous mutants (HOM2,4,7) that were both heterozygous for the FoxG1 transcription factor, as Cre recombinase expression is driven off the endogenous FoxG1 locus in these animals (Hebert, J. M., Lin, M., Partanen, J., Rossant, J. and McConnell, S. K. (2003b). FGF signaling through FGFR1 is required for olfactory bulb morphogenesis. Development 130, 1101-11. & Hebert, J. M. and McConnell, S. K. (2000). Targeting of cre to the Foxg1 (BF-1) locus mediates loxP recombination in the telencephalon and other developing head structures. Dev Biol 222, 296-306.
Data processing log ratios were lowess normalised, and filtered to exclude (1) spots flagged as bad and (2) spots where 55% of the foreground pixels in either channel were less intense than the (background intensity + 1SD)
 
Submission date Jun 30, 2005
Last update date Aug 26, 2005
Contact name Stephen Sansom
E-mail(s) stephen.sansom@kennedy.ox.ac.uk
Organization name Kennedy Institute of Rheumatology
Department NDORMS
Lab Sansom
Street address Roosevelt Drive
City Oxford
State/province Oxfordshire
ZIP/Postal code OX3 7FY
Country United Kingdom
 
Platform ID GPL2584
Series (1)
GSE2857 Comparison of gene expression in FGFR1 heterozygous and null E12.5 murine telencephalons

Data table header descriptions
ID_REF
VALUE Log ratio, lowess normalised, background filtered

Data table
ID_REF VALUE
CG_Mm_3001006_1 -0.548
CG_Mm_EXT_3110714_1 -0.193
CG_MmCTRL_30001_02 null
CG_MmCTRL_30001_03 null
CG_MmCTRL_30001_04 null
CG_MmCTRL_30001_05 null
CG_MmCTRL_30001_06 null
CG_MmCTRL_30001_07 null
CG_MmCTRL_30001_08 null
CG_MmCTRL_30001_09 null
CG_MmCTRL_30001_10 null
CG_MmCTRL_30001_100 null
CG_MmCTRL_30001_101 null
CG_MmCTRL_30001_102 null
CG_MmCTRL_30001_103 null
CG_MmCTRL_30001_104 null
CG_MmCTRL_30001_105 null
CG_MmCTRL_30001_106 null
CG_MmCTRL_30001_107 null
CG_MmCTRL_30001_108 null

Total number of rows: 21998

Table truncated, full table size 534 Kbytes.




Supplementary data files not provided

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