|
Status |
Public on Jul 05, 2006 |
Title |
right atrium, obese 3-lean 4 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
right atrium, obese-3
|
Organism |
Rattus norvegicus |
Characteristics |
Strain : SHHF, Gender: Male, Age: 10 months old, Tissue: right atrium, Other: hypertension, metabolic syndrom, dilated cardiomyopathy starting from 10 months old
|
Biomaterial provider |
Charles River laboratory
|
Treatment protocol |
None
|
Growth protocol |
Animals were lodged in the Zootechnic service of the Louis Bugnard Institute. A veterinary oversees animal health and management. Temperature : 22 +/- 1 degrees. Cycles nights/days : 12 hours. Food : Teklad Global 16% Protein Rodent Diet (Harlan)
|
Extracted molecule |
total RNA |
Extraction protocol |
General anesthesia by Isoflurane 4%. Heart extraction. Isolation of total RNA from left ventricular by : TRIzol (Invitrogen), homogeneisor Ika Ultra-Thurax T 25 (Janke and Kunkel), DNAfree (Ambion). Quality and quantity were determined by capillary electrophoresis (Agilent).
|
Label |
Cy3
|
Label protocol |
Reverse Transcription of 5 µg total RNA from each right atrium with Superscript enzyme (Invitrogen). Using of Cy3-dCTP from Amersham Society
|
|
|
Channel 2 |
Source name |
right atrium, lean 4
|
Organism |
Rattus norvegicus |
Characteristics |
Strain : SHHF, Gender: Male, Age: 10 months old, Tissue: right atrium, Other: hypertension, dilated cardiomyopathy starting from 14 months old
|
Biomaterial provider |
Charles River laboratory
|
Treatment protocol |
None
|
Growth protocol |
Animals were lodged in the Zootechnic service of the Louis Bugnard Institute. A veterinary oversees animal health and management. Temperature : 22 +/- 1 degrees. Cycles nights/days : 12 hours. Food : Teklad Global 16% Protein Rodent Diet (Harlan)
|
Extracted molecule |
total RNA |
Extraction protocol |
General anesthesia by Isoflurane 4%. Heart extraction. Isolation of total RNA from left ventricular by : TRIzol (Invitrogen), homogeneisor Ika Ultra-Thurax T 25 (Janke and Kunkel), DNAfree (Ambion). Quality and quantity were determined by capillary electrophoresis (Agilent).
|
Label |
Cy5
|
Label protocol |
Reverse Transcription of 5 µg total RNA from each right atrium with Superscript enzyme (Invitrogen). Using of Cy5-dCTP from Amersham Society
|
|
|
|
Hybridization protocol |
kit Pronto plus System 6 (Corning Incorporated and Promega Corporation)
|
Scan protocol |
Scan with Genepix 4100 A (Axon Instrument). Image analysis by Genepix version 6 (Axon Instrument).
|
Description |
Lean rats can be homozygous or heterozygous for the leptin receptor gene (+/+ or cp/+, cp means corpulent allele and is a recessive autosomic one, + means wild type)
|
Data processing |
background subtraction, log transformation, normalization by total mean
|
|
|
Submission date |
Jul 04, 2005 |
Last update date |
Sep 22, 2005 |
Contact name |
Philippe V Rouet |
E-mail(s) |
Philippe.rouet@inserm.fr
|
Phone |
00 33 5 31 22 41 19
|
Organization name |
INSERM U1048
|
Department |
Cardiology
|
Lab |
Team 7
|
Street address |
1 Ave Jean Poulhes
|
City |
Toulouse |
State/province |
BP84225 |
ZIP/Postal code |
31432 |
Country |
France |
|
|
Platform ID |
GPL2594 |
Series (1) |
GSE2877 |
Impact of the obesity on the right atrium transcriptom of SHHF rats |
|