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Sample GSM6297347 Query DataSets for GSM6297347
Status Public on Feb 26, 2024
Title Growth Factor-induced hiPSC-derived HLCs Day 4 [GC117476]
Sample type SRA
 
Source name reprogrammed epithelial cells
Organism Homo sapiens
Characteristics tissue: reprogrammed epithelial cells
cell line: Sigma 3X (induced pluripotent stem cells)
cell type: iPSC0028 (Sigma-Aldrich) genome engineered
cell subtype: endoderm
time point: Day 4
Extracted molecule total RNA
Extraction protocol RNA was extracted and quality control was performed as mentioned above.We performed RTqPCR, using progeny from 3 wells of 96 well plates for RNA extraction using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer’s instructions with addition of DNAseI from the On-Column DNase I Digestion kit (Sigma-Aldrich).
Library preparation was performed with the LexoGen Quant Seq. Kit. Libraries were sequenced on an Illumina HiSeq4000 sequencing system.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Data processing Single-end reads were generated, with an average of 4 million reads obtained per sample. Alignment was performed with Hisat2. Raw counts were log2 transformed before performing downstream analyses.
Assembly: hg18
Supplementary files format and content: tab-delimited text file includes number of transcripts for each sample
Supplementary files format and content: comparison between different samples, includes tab-delimited text file for all samples
 
Submission date Jul 06, 2022
Last update date Feb 26, 2024
Contact name Gert Vanmarcke
E-mail(s) gert.vanmarcke@kuleuven.be
Organization name KU Leuven
Department Department of Development and Regeneration
Lab Stem Cell Institute
Street address Herestraat 49
City Leuven
ZIP/Postal code 3000
Country Belgium
 
Platform ID GPL20301
Series (1)
GSE207569 Automated generation of hiPSC-derived hepatic progeny by cost-efficient compounds
Relations
BioSample SAMN29535229
SRA SRX16038466

Supplementary file Size Download File type/resource
GSM6297347_GC117476.count.txt.gz 191.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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