|
| Status |
Public on Jan 01, 2006 |
| Title |
cDNA array +10wt/+10fl set3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
+10 DPA wide-type cotton ovule
|
| Organism |
Gossypium hirsutum |
| Characteristics |
Extracted total RNA from +10 DPA wide-type cotton ovules
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| Channel 2 |
| Source name |
+10 DPA fl-mutant cotton ovule
|
| Organism |
Gossypium hirsutum |
| Characteristics |
Extracted total RNA from +10 DPA fl-mutant cotton ovules
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| |
| Description |
Comparison of gene expression profiles of +10 DPA wt and +10 DPA fl-mutant upland cotton ovules
|
| Data processing |
All microarrays were scanned with a ScanArray Express scanner using the ScanArray 2.0 software (Packard Bioscience, Meriden, CT) and analyzed using the GenePix Pro 4.0 software (Axon Instruments, Foster City, CA). We quantified signal intensities of individual spots from the 16-bit TIFF images using GenePix Pro 4.0 (Axon Instruments). Since extensive expression pattern shift was recorded in mRNA populations of late developmental stages, such as the 10dpa/3dpa sample pair shown in Fig. 1C, we applied a linear normalization strategy (van de Peppel et al., 2003) instead of the nonlinear global-intensity-based LOWESS program used for most genome-wide microarrays (Yang et al., 2002). Expression ratios were collected only on those spots with signal intensity >800 in at least one dye channel on the microarray slides. Experimental parameters will be included in supplementary file.
|
| |
|
| Submission date |
Jul 11, 2005 |
| Last update date |
Dec 26, 2005 |
| Contact name |
Yu-Xian Zhu |
| E-mail(s) |
zhuyx@water.pku.edu.cn
|
| Phone |
86-10-62751193
|
| Fax |
86-10-62754427
|
| Organization name |
College of life sciences, Peking University
|
| Lab |
National Lab of Protein Engineering & Plant Genetic Engineering
|
| Street address |
5# Summer Palace Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
| |
|
| Platform ID |
GPL2610 |
| Series (1) |
| GSE2901 |
cDNA array expression profile of wild-type and fl-mutant upland cotton ovules |
|
