|
Status |
Public on Jul 22, 2022 |
Title |
No insomina disorder [930c13017] |
Sample type |
RNA |
|
|
Source name |
PBMCs
|
Organism |
Homo sapiens |
Characteristics |
insomnia: no age: 77 gender: male race: white education (years): 12 bmi: 36.03 comorbidity: 0 bdi: 2 bdins: 2 history of depression: yes
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from PMBC samples lysed in RLT buffer following the protocol from a QIAGEN RNeasy mini kit on a QIACube instrument and included DNase I treatment.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with Ambion TotalPrep for Illumina arrays (carried out by UCLA Neuroscience Genomics Core).
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol (carried out by UCLA Neuroscience Genomics Core).
|
Scan protocol |
Standard Illumina scan protocol for gene expression profiling (carried out by UCLA Neuroscience Genomics Core).
|
Description |
Illumina HumanHT-12 v4.0 expression beadchip
|
Data processing |
Raw probe intensity values and quantile-normalized gene expression values were generated by Illumina GenomeStudio software using Gene Expression module 1.1.1. Data were then log2 transformed before analysis
|
|
|
Submission date |
Jul 20, 2022 |
Last update date |
Jul 22, 2022 |
Contact name |
Donald M. Lamkin |
E-mail(s) |
dlamkin@ucla.edu
|
Organization name |
UCLA
|
Department |
Semel Institute for Neuroscience
|
Street address |
300 UCLA Medical Plaza, Room 3160
|
City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
|
|
Platform ID |
GPL10904 |
Series (1) |
GSE208668 |
Sleep Disturbance and Activation of Cellular and Transcriptional Mechanisms of Inflammation in Older Adults |
|