|
| Status |
Public on Jul 22, 2022 |
| Title |
No insomina disorder [930c14402] |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
insomnia: no
age: 62
gender: male
race: white
education (years): 24
bmi: 24.9
comorbidity: 0
bdi: 5
bdins: 5
history of depression: yes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from PMBC samples lysed in RLT buffer following the protocol from a QIAGEN RNeasy mini kit on a QIACube instrument and included DNase I treatment.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Ambion TotalPrep for Illumina arrays (carried out by UCLA Neuroscience Genomics Core).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol (carried out by UCLA Neuroscience Genomics Core).
|
| Scan protocol |
Standard Illumina scan protocol for gene expression profiling (carried out by UCLA Neuroscience Genomics Core).
|
| Description |
Illumina HumanHT-12 v4.0 expression beadchip
|
| Data processing |
Raw probe intensity values and quantile-normalized gene expression values were generated by Illumina GenomeStudio software using Gene Expression module 1.1.1. Data were then log2 transformed before analysis
|
| |
|
| Submission date |
Jul 20, 2022 |
| Last update date |
Jul 22, 2022 |
| Contact name |
Donald M. Lamkin |
| E-mail(s) |
dlamkin@ucla.edu
|
| Organization name |
UCLA
|
| Department |
Semel Institute for Neuroscience
|
| Street address |
300 UCLA Medical Plaza, Room 3160
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL10904 |
| Series (1) |
| GSE208668 |
Sleep Disturbance and Activation of Cellular and Transcriptional Mechanisms of Inflammation in Older Adults |
|
