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Sample GSM637863 Query DataSets for GSM637863
Status Public on Dec 10, 2010
Title Sample 10 wildtype
Sample type RNA
 
Source name E9.5 anterior neural tube
Organism Mus musculus
Characteristics strain: C57BL/6 and 129S5/SvEvBrd (B6;129S5)
genotype/variation: WT
tissue: posterior neural tube
age: embryo day 9.5
Treatment protocol The posterior part of the embryo (immediately below the forelimb pit) was cut off and transferred separately to a 600 uL plastic tube containing 15 uL of RNAlater (Ambion).
Growth protocol Embryos were removed from pregnant dams at embryonic day 9.5. At E 9.5, the average embryo was ā€œCā€ shaped and had 21.6 pairs of somites and neurul tube closure was complete with the exception of the posterior neuropore.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted from collected posterior parts of E9.5 embryos using PicoPure RNA isolation Kit (Arcturus).
Label biotin-UTP
Label protocol RNA that has passed the Agilent Technologies 2100 Bioanalyzer quality control test was used to generate biotin-labeled cRNA via a modified Eberwine RNA amplification protocol
 
Hybridization protocol Hybridization was according Applied Microarrays Specifications. Detection of biotin label used Alexa-Fluro
Scan protocol Agilent DNA Microarray Scanner
Description 36227 oligo probes, CodeLink Mouse Whole Genome Bioarray
T00317570
Data processing Initial feature extraction from the images was performed using CodeLink Expression Analysis software v4.0. Median-Normalization.
 
Submission date Dec 09, 2010
Last update date Dec 10, 2010
Contact name Robert Cabrera
Organization name DPRI
Street address 1400 Barbara Jordan Blvd
City Austin
State/province TX
ZIP/Postal code 78723
Country USA
 
Platform ID GPL2897
Series (2)
GSE25974 Gene expression-based analysis of neural tube closure for the posterior neuropore of Fkbp8 embryos at E9.5
GSE25983 Gene expression-based analysis of neural tube closure for the posterior neuropore

Data table header descriptions
ID_REF
VALUE Normalized Signal Intensities

Data table
ID_REF VALUE
301077 0.843121178
301091 57.15053449
383035 1.27421684
383049 1.419458541
383091 7.014666978
383105 6.135665724
56001 0.575216475
56015 16.55864949
56085 18.15447075
220057 12.25513925
302001 21.92371754
302029 9.15610185
302043 6.798740049
302057 27.1372809
302071 47.99793184
302085 7.183278251
302099 15.96529231
384001 3.907452672
384015 12.37121264
384029 0.126093051

Total number of rows: 36227

Table truncated, full table size 656 Kbytes.




Supplementary file Size Download File type/resource
GSM637863_T00317570_NegativeControlSummary.txt.gz 3.6 Kb (ftp)(http) TXT
GSM637863_T00317570_PositiveControlSummary.txt.gz 3.5 Kb (ftp)(http) TXT
GSM637863_T00317570_SAMPLE_10wildtype.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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