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Sample GSM637866 Query DataSets for GSM637866
Status Public on Dec 10, 2010
Title SAMPLE005 wildtype
Sample type RNA
 
Source name E9.5 anterior neural tube
Organism Mus musculus
Characteristics strain: LMBc
genotype/variation: WT
tissue: posterior neural tube
age: embryo day 9.5
Treatment protocol The posterior part of the embryo (immediately below the forelimb pit) was cut off and transferred separately to a 600 uL plastic tube containing 15 uL of RNAlater (Ambion).
Growth protocol Embryos were removed from pregnant dams at embryonic day 9.5. At E 9.5, the average embryo was ā€œCā€ shaped and had 21.3 pairs of somites and neurul tube closure was complete with the exception of the posterior neuropore.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted from collected posterior parts of E9.5 embryos using PicoPure RNA isolation Kit (Arcturus).
Label biotin-UTP
Label protocol RNA that has passed the Agilent Technologies 2100 Bioanalyzer quality control test was used to generate biotin-labeled cRNA via a modified Eberwine RNA amplification protocol
 
Hybridization protocol Hybridization was according Applied Microarrays Specifications. Detection of biotin label used Alexa-Fluro
Scan protocol Agilent DNA Microarray Scanner
Description 36227 oligo probes, CodeLink Mouse Whole Genome Bioarray
T00341107
Data processing Initial feature extraction from the images was performed using CodeLink Expression Analysis software v4.0. Global-Median-Normalization.
 
Submission date Dec 09, 2010
Last update date Dec 10, 2010
Contact name Robert Cabrera
Organization name DPRI
Street address 1400 Barbara Jordan Blvd
City Austin
State/province TX
ZIP/Postal code 78723
Country USA
 
Platform ID GPL2897
Series (2)
GSE25975 Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5
GSE25983 Gene expression-based analysis of neural tube closure for the posterior neuropore

Data table header descriptions
ID_REF
VALUE Normalized Signal Intensities

Data table
ID_REF VALUE
301077 1.057752674
301091 65.60312631
383035 1.179668455
383049 1.039446826
383091 4.860047038
383105 14.46573547
56001 0.389834451
56015 17.72412394
56085 26.28433468
220057 18.65869754
302001 81.61879744
302029 9.339539807
302043 11.2847759
302057 34.81619865
302071 76.28059996
302085 4.201221507
302099 26.63254834
384001 19.69869768
384015 11.81053313
384029 0.061941946

Total number of rows: 36227

Table truncated, full table size 654 Kbytes.




Supplementary file Size Download File type/resource
GSM637866_T00341107_NegativeControlSummary.txt.gz 3.0 Kb (ftp)(http) TXT
GSM637866_T00341107_PositiveControlSummary.txt.gz 3.6 Kb (ftp)(http) TXT
GSM637866_T00341107_SAMPLE005-wt.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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