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Sample GSM637868 Query DataSets for GSM637868
Status Public on Dec 10, 2010
Title SAMPLE009 null
Sample type RNA
 
Source name E9.5 anterior neural tube
Organism Mus musculus
Characteristics strain: LMBc
genotype/variation: Splotch null
tissue: posterior neural tube
age: embryo day 9.5
Treatment protocol The posterior part of the embryo (immediately below the forelimb pit) was cut off and transferred separately to a 600 uL plastic tube containing 15 uL of RNAlater (Ambion).
Growth protocol Embryos were removed from pregnant dams at embryonic day 9.5. At E 9.5, the average embryo was ā€œCā€ shaped and had 21.3 pairs of somites and neurul tube closure was complete with the exception of the posterior neuropore.
Extracted molecule total RNA
Extraction protocol The total RNA was extracted from collected posterior parts of E9.5 embryos using PicoPure RNA isolation Kit (Arcturus).
Label biotin-UTP
Label protocol RNA that has passed the Agilent Technologies 2100 Bioanalyzer quality control test was used to generate biotin-labeled cRNA via a modified Eberwine RNA amplification protocol
 
Hybridization protocol Hybridization was according Applied Microarrays Specifications. Detection of biotin label used Alexa-Fluro
Scan protocol Agilent DNA Microarray Scanner
Description 36227 oligo probes, CodeLink Mouse Whole Genome Bioarray
T00341109
Data processing Initial feature extraction from the images was performed using CodeLink Expression Analysis software v4.0. Global-Median-Normalization.
 
Submission date Dec 09, 2010
Last update date Dec 10, 2010
Contact name Robert Cabrera
Organization name DPRI
Street address 1400 Barbara Jordan Blvd
City Austin
State/province TX
ZIP/Postal code 78723
Country USA
 
Platform ID GPL2897
Series (2)
GSE25975 Gene expression-based analysis of neural tube closure for the posterior neuropore of Splotch embryos at E9.5
GSE25983 Gene expression-based analysis of neural tube closure for the posterior neuropore

Data table header descriptions
ID_REF
VALUE Normalized Signal Intensities

Data table
ID_REF VALUE
301077 0.657380377
301091 72.06039415
383035 1.074372472
383049 1.159732357
383091 7.045431637
383105 19.07728009
56001 0.530683782
56015 22.90321622
56085 27.66979256
220057 21.1647421
302001 80.37257927
302029 9.910214594
302043 12.62859309
302057 45.81883372
302071 60.30564248
302085 11.30115274
302099 27.88344788
384001 16.79999221
384015 18.7581209
384029 0.177034439

Total number of rows: 36227

Table truncated, full table size 654 Kbytes.




Supplementary file Size Download File type/resource
GSM637868_T00341109_NegativeControlSummary.txt.gz 3.0 Kb (ftp)(http) TXT
GSM637868_T00341109_PositiveControlSummary.txt.gz 3.6 Kb (ftp)(http) TXT
GSM637868_T00341109_SAMPLE009.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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